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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12453 | 5' | -58.4 | NC_003324.1 | + | 31697 | 1.06 | 0.000741 |
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Target: 5'- cAUCCCCGUGCUGGUGACGCGAACCACc -3' miRNA: 3'- -UAGGGGCACGACCACUGCGCUUGGUG- -5' |
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| 12453 | 5' | -58.4 | NC_003324.1 | + | 20812 | 0.75 | 0.155419 |
|
Target: 5'- ---aCCGUGUUGGUGACGCGcAUCGCc -3' miRNA: 3'- uaggGGCACGACCACUGCGCuUGGUG- -5' |
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| 12453 | 5' | -58.4 | NC_003324.1 | + | 32503 | 0.7 | 0.304944 |
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Target: 5'- cAUCaagaaCCGUGCUGGUGuGCGCGGuaagagcgcGCCGa -3' miRNA: 3'- -UAGg----GGCACGACCAC-UGCGCU---------UGGUg -5' |
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| 12453 | 5' | -58.4 | NC_003324.1 | + | 27276 | 0.69 | 0.377738 |
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Target: 5'- -cUUCCGcGUUGGUGGCGCGGAuuucCCGCc -3' miRNA: 3'- uaGGGGCaCGACCACUGCGCUU----GGUG- -5' |
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| 12453 | 5' | -58.4 | NC_003324.1 | + | 49098 | 0.67 | 0.471228 |
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Target: 5'- cGUCUauuUCGcUGCcGGUGACGCGAucGCCAa -3' miRNA: 3'- -UAGG---GGC-ACGaCCACUGCGCU--UGGUg -5' |
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| 12453 | 5' | -58.4 | NC_003324.1 | + | 38103 | 0.66 | 0.501509 |
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Target: 5'- -gCgCCGUGCUGGUGACcguucuugugggGCGuuucgGACCAg -3' miRNA: 3'- uaGgGGCACGACCACUG------------CGC-----UUGGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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