Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12454 | 3' | -64.7 | NC_003324.1 | + | 14570 | 0.67 | 0.242351 |
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Target: 5'- -aUGGGucgcGGCcuGCCGGCGCaagcgGCCGGCg -3' miRNA: 3'- ugACCC----CCG--CGGUUGCGga---CGGCCGg -5' |
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| 12454 | 3' | -64.7 | NC_003324.1 | + | 14423 | 0.67 | 0.230174 |
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Target: 5'- -aUGGGGaucaCGCCcggcucuGGCGCCcaUGCCGGCg -3' miRNA: 3'- ugACCCCc---GCGG-------UUGCGG--ACGGCCGg -5' |
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| 12454 | 3' | -64.7 | NC_003324.1 | + | 10677 | 0.68 | 0.20893 |
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Target: 5'- gACUGGGGGacgguuGCCGuUGCCUGCauccGCUg -3' miRNA: 3'- -UGACCCCCg-----CGGUuGCGGACGgc--CGG- -5' |
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| 12454 | 3' | -64.7 | NC_003324.1 | + | 7631 | 0.67 | 0.254443 |
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Target: 5'- --cGGaGGCGU--GCGCCuUGCUGGCCc -3' miRNA: 3'- ugaCCcCCGCGguUGCGG-ACGGCCGG- -5' |
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| 12454 | 3' | -64.7 | NC_003324.1 | + | 3636 | 0.73 | 0.093217 |
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Target: 5'- -aUGGcGGGCGCCAccgaaGCGCCguuugcaGCCGGUUc -3' miRNA: 3'- ugACC-CCCGCGGU-----UGCGGa------CGGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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