Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12454 | 3' | -64.7 | NC_003324.1 | + | 14696 | 0.67 | 0.236487 |
Target: 5'- --aGGGccguagcuccGGcCGCCAcgcuaccgccuGCGCC-GCCGGCCg -3' miRNA: 3'- ugaCCC----------CC-GCGGU-----------UGCGGaCGGCCGG- -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 30310 | 0.67 | 0.242351 |
Target: 5'- --cGGuGGCGUCGcucgGCGCgUGCUGGCUg -3' miRNA: 3'- ugaCCcCCGCGGU----UGCGgACGGCCGG- -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 7631 | 0.67 | 0.254443 |
Target: 5'- --cGGaGGCGU--GCGCCuUGCUGGCCc -3' miRNA: 3'- ugaCCcCCGCGguUGCGG-ACGGCCGG- -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 41818 | 0.66 | 0.286836 |
Target: 5'- gUUGGGGGUGCCGugGCgUGaucGCa -3' miRNA: 3'- uGACCCCCGCGGUugCGgACggcCGg -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 38988 | 0.73 | 0.090748 |
Target: 5'- -aUGGaGGCGCUGGCGCUUGCCgaGGCUg -3' miRNA: 3'- ugACCcCCGCGGUUGCGGACGG--CCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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