Results 1 - 20 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12456 | 3' | -53.3 | NC_003324.1 | + | 29954 | 0.66 | 0.80715 |
Target: 5'- ----cGAggGGCGUccUGcGUAGCGGCGGCa -3' miRNA: 3'- aagaaCUa-CUGCG--AC-CGUUGCCGUCG- -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 51542 | 0.66 | 0.797277 |
Target: 5'- aUCgauaUGAUGccaACuCUGGUGACGGaCAGCa -3' miRNA: 3'- aAGa---ACUAC---UGcGACCGUUGCC-GUCG- -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 14522 | 0.66 | 0.787221 |
Target: 5'- -gCUUG--GACGCcGGCAugGGCccagAGCc -3' miRNA: 3'- aaGAACuaCUGCGaCCGUugCCG----UCG- -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 13161 | 0.66 | 0.787221 |
Target: 5'- ----cGgcGGCGgUcGGCGGCGGCGGUa -3' miRNA: 3'- aagaaCuaCUGCgA-CCGUUGCCGUCG- -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 2521 | 0.66 | 0.787221 |
Target: 5'- --gUUGGUGGggaacaagcCGCcGGCGuCGGCGGCu -3' miRNA: 3'- aagAACUACU---------GCGaCCGUuGCCGUCG- -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 50431 | 0.66 | 0.787221 |
Target: 5'- -------aGGCGCUgGGCGugGuGCAGCu -3' miRNA: 3'- aagaacuaCUGCGA-CCGUugC-CGUCG- -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 17560 | 0.66 | 0.776995 |
Target: 5'- cUCUcucaGACGCUcGGCGcgACGGCAGg -3' miRNA: 3'- aAGAacuaCUGCGA-CCGU--UGCCGUCg -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 35604 | 0.66 | 0.776995 |
Target: 5'- cUUCUUGccGAUGUcGGCuucugcuGCGcGCAGCa -3' miRNA: 3'- -AAGAACuaCUGCGaCCGu------UGC-CGUCG- -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 45357 | 0.66 | 0.776995 |
Target: 5'- -aCUUGAa---GCaGGCGGCGaGCAGCg -3' miRNA: 3'- aaGAACUacugCGaCCGUUGC-CGUCG- -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 17172 | 0.66 | 0.756076 |
Target: 5'- --aUUGAcgaGGCGCUGGCGcgucugucggacGCGGCuuucGGCg -3' miRNA: 3'- aagAACUa--CUGCGACCGU------------UGCCG----UCG- -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 23990 | 0.67 | 0.74541 |
Target: 5'- -gCUcGAc-GCGCUGGUAggGCGGCGGUa -3' miRNA: 3'- aaGAaCUacUGCGACCGU--UGCCGUCG- -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 10588 | 0.67 | 0.723724 |
Target: 5'- ----aGcgGAUGCaGGCAACGGCAa- -3' miRNA: 3'- aagaaCuaCUGCGaCCGUUGCCGUcg -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 24077 | 0.67 | 0.723724 |
Target: 5'- cUCgccGGUG-CGCUGcGCcACGGUGGCg -3' miRNA: 3'- aAGaa-CUACuGCGAC-CGuUGCCGUCG- -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 45208 | 0.67 | 0.723724 |
Target: 5'- gUUgUUGAU-ACGCcGGCAGCGGUcgagAGCc -3' miRNA: 3'- -AAgAACUAcUGCGaCCGUUGCCG----UCG- -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 2682 | 0.67 | 0.720436 |
Target: 5'- ----cGGUGGcCGCUucGGCAagggcacgaugucgGCGGCGGCa -3' miRNA: 3'- aagaaCUACU-GCGA--CCGU--------------UGCCGUCG- -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 25265 | 0.67 | 0.701652 |
Target: 5'- gUCUcaugccUGGUGA-GCUGcGCGACGGC-GCc -3' miRNA: 3'- aAGA------ACUACUgCGAC-CGUUGCCGuCG- -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 21938 | 0.68 | 0.679293 |
Target: 5'- cUUCUUGugGAUGCgGGCcucucguCGGCGGCg -3' miRNA: 3'- -AAGAACuaCUGCGaCCGuu-----GCCGUCG- -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 44566 | 0.68 | 0.679293 |
Target: 5'- ---gUGAUGAcccCGUUcGGCAgcgacACGGCGGCg -3' miRNA: 3'- aagaACUACU---GCGA-CCGU-----UGCCGUCG- -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 33348 | 0.68 | 0.679293 |
Target: 5'- ----cGAUGACuaUGGCGAUGGCGu- -3' miRNA: 3'- aagaaCUACUGcgACCGUUGCCGUcg -5' |
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12456 | 3' | -53.3 | NC_003324.1 | + | 35193 | 0.68 | 0.668037 |
Target: 5'- ----cGAcGGCGCUGGCGacgACGGaaaGGCa -3' miRNA: 3'- aagaaCUaCUGCGACCGU---UGCCg--UCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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