miRNA: 3'- aUGCGGAC--GCGACgGCGGUUCCGUag -5'
miRNA: 3'- -AUGCGGA-CGCGACGg-CGGuucCGuAG- -5'
miRNA: 3'- augCGGACgCGACGGCGGuu--CCGUag -5'
miRNA: 3'- aUGCGGa---CGCGA---------------CGGCGGU-UC-CGUAG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12458 | 3' | -60.3 | NC_003324.1 | + | 25097 | 0.67 | 0.345751 |
|
Target: 5'- gGCGUCUGgaUGUUGgCGCCGGGGUAg- -3' miRNA: 3'- aUGCGGAC--GCGACgGCGGUUCCGUag -5' |
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| 12458 | 3' | -60.3 | NC_003324.1 | + | 31137 | 0.67 | 0.345751 |
|
Target: 5'- uUGCGCCUgGCGCUGCagGCCu--GCcUCg -3' miRNA: 3'- -AUGCGGA-CGCGACGg-CGGuucCGuAG- -5' |
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| 12458 | 3' | -60.3 | NC_003324.1 | + | 51539 | 0.67 | 0.345751 |
|
Target: 5'- --gGCUUGgGCUGCCGUCuugcGGCAa- -3' miRNA: 3'- augCGGACgCGACGGCGGuu--CCGUag -5' |
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| 12458 | 3' | -60.3 | NC_003324.1 | + | 21499 | 0.67 | 0.365862 |
|
Target: 5'- cGCGCCcgucGCGUUgcucaaggucggacgGCCGCCAcGGcGCAUCg -3' miRNA: 3'- aUGCGGa---CGCGA---------------CGGCGGU-UC-CGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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