Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12459 | 3' | -50.6 | NC_003324.1 | + | 14569 | 0.66 | 0.922975 |
Target: 5'- ---cAUgGGUcGCGGCcUGCcggcgcaaGCGGCCg -3' miRNA: 3'- gauaUAgCUAaCGCCGaACG--------UGCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 6786 | 0.66 | 0.922975 |
Target: 5'- ---cAUCGAUguCGGCUUGCccgaacccuacaGCGuGCCg -3' miRNA: 3'- gauaUAGCUAacGCCGAACG------------UGC-CGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 19864 | 0.67 | 0.903354 |
Target: 5'- -----gCGaAUUGCGGCUU-UACGGUCa -3' miRNA: 3'- gauauaGC-UAACGCCGAAcGUGCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 25608 | 0.67 | 0.896233 |
Target: 5'- --cUGUCGGccGCuGCUgcgccgGCACGGCUg -3' miRNA: 3'- gauAUAGCUaaCGcCGAa-----CGUGCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 38009 | 0.67 | 0.888828 |
Target: 5'- -----gCGGUUGCGGC--GCACGGg- -3' miRNA: 3'- gauauaGCUAACGCCGaaCGUGCCgg -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 43470 | 0.68 | 0.854758 |
Target: 5'- ---gAUCGAgcaacUUGCGGCcacgguucaugaUGCGCGGCa -3' miRNA: 3'- gauaUAGCU-----AACGCCGa-----------ACGUGCCGg -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 19979 | 0.69 | 0.82015 |
Target: 5'- ---aGUCGAUUaUGGCg-GCGCGGUCa -3' miRNA: 3'- gauaUAGCUAAcGCCGaaCGUGCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 32036 | 0.69 | 0.82015 |
Target: 5'- gCUGUcugcCGGcgGCGGCU--CACGGCCg -3' miRNA: 3'- -GAUAua--GCUaaCGCCGAacGUGCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 44308 | 0.69 | 0.800677 |
Target: 5'- -----cCGGgcGCGGUg-GCGCGGCCg -3' miRNA: 3'- gauauaGCUaaCGCCGaaCGUGCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 10014 | 0.69 | 0.780453 |
Target: 5'- -gAUAaCGAcgGCGGCcgaaaucaagaaUUGUGCGGCCu -3' miRNA: 3'- gaUAUaGCUaaCGCCG------------AACGUGCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 12102 | 0.7 | 0.759572 |
Target: 5'- uCUGUAccUGAUcGCGGagcGCGCGGCCu -3' miRNA: 3'- -GAUAUa-GCUAaCGCCgaaCGUGCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 49530 | 0.74 | 0.525255 |
Target: 5'- -cGUAUCGA--GCGGCgaggcgacgUGCAUGGUCg -3' miRNA: 3'- gaUAUAGCUaaCGCCGa--------ACGUGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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