Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12459 | 5' | -48.8 | NC_003324.1 | + | 36936 | 0.67 | 0.951442 |
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Target: 5'- aGCGuccGUCGUcGAGGUCCAgcUCGGUc- -3' miRNA: 3'- -CGU---UAGCAuCUCCAGGUguAGCUAca -5' |
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| 12459 | 5' | -48.8 | NC_003324.1 | + | 43410 | 0.67 | 0.946721 |
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Target: 5'- cGCAGUCGUGGGuGGUCUcgGCGacggCGAaauUGUa -3' miRNA: 3'- -CGUUAGCAUCU-CCAGG--UGUa---GCU---ACA- -5' |
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| 12459 | 5' | -48.8 | NC_003324.1 | + | 47470 | 0.68 | 0.930791 |
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Target: 5'- gGCGAUgCGUAGAuGGUCCuGCAUCa---- -3' miRNA: 3'- -CGUUA-GCAUCU-CCAGG-UGUAGcuaca -5' |
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| 12459 | 5' | -48.8 | NC_003324.1 | + | 41918 | 0.69 | 0.90537 |
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Target: 5'- cGCAGUCGccgcauccGGAGGUaCCGCGUuccCGAUGc -3' miRNA: 3'- -CGUUAGCa-------UCUCCA-GGUGUA---GCUACa -5' |
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| 12459 | 5' | -48.8 | NC_003324.1 | + | 47161 | 0.7 | 0.858558 |
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Target: 5'- cGCAcgUGUcGGGGUCUACuuggCGAUGg -3' miRNA: 3'- -CGUuaGCAuCUCCAGGUGua--GCUACa -5' |
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| 12459 | 5' | -48.8 | NC_003324.1 | + | 41436 | 0.71 | 0.830659 |
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Target: 5'- aGCccUCGUAGAGGUCCcaaaccgaaggcgGCAUUGGUa- -3' miRNA: 3'- -CGuuAGCAUCUCCAGG-------------UGUAGCUAca -5' |
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| 12459 | 5' | -48.8 | NC_003324.1 | + | 29959 | 0.71 | 0.812465 |
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Target: 5'- gGCGuccugCGUAGcggcggcagccGGGUCCGCAUCGAUc- -3' miRNA: 3'- -CGUua---GCAUC-----------UCCAGGUGUAGCUAca -5' |
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| 12459 | 5' | -48.8 | NC_003324.1 | + | 33849 | 1.11 | 0.003846 |
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Target: 5'- gGCAAUCGUAGAGGUCCACAUCGAUGUa -3' miRNA: 3'- -CGUUAGCAUCUCCAGGUGUAGCUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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