Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12460 | 3' | -58.7 | NC_003324.1 | + | 42368 | 0.66 | 0.512634 |
Target: 5'- aGGCg-GCGCACUCAgcguUGCCgacccgcgCCCUGUa -3' miRNA: 3'- -UUGagCGCGUGAGU----GCGGa-------GGGACAg -5' |
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12460 | 3' | -58.7 | NC_003324.1 | + | 43681 | 0.66 | 0.502306 |
Target: 5'- gAACUCGCcauGCGCUgCGCGCCgaa-UGUCg -3' miRNA: 3'- -UUGAGCG---CGUGA-GUGCGGagggACAG- -5' |
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12460 | 3' | -58.7 | NC_003324.1 | + | 20277 | 0.66 | 0.481933 |
Target: 5'- uAC-CGgGCGCguuacgCGCGCCUCUCUGa- -3' miRNA: 3'- uUGaGCgCGUGa-----GUGCGGAGGGACag -5' |
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12460 | 3' | -58.7 | NC_003324.1 | + | 13470 | 0.68 | 0.404855 |
Target: 5'- uGCUCGcCGaugucaaagaGCUUaauaaGCGCCUCUCUGUCa -3' miRNA: 3'- uUGAGC-GCg---------UGAG-----UGCGGAGGGACAG- -5' |
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12460 | 3' | -58.7 | NC_003324.1 | + | 40990 | 0.68 | 0.378017 |
Target: 5'- aGGCUCGCGCGCUCA--UCUCUgaGUUg -3' miRNA: 3'- -UUGAGCGCGUGAGUgcGGAGGgaCAG- -5' |
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12460 | 3' | -58.7 | NC_003324.1 | + | 7846 | 0.69 | 0.34414 |
Target: 5'- --gUUGCGa--UCGCGCCUCCCUG-Cg -3' miRNA: 3'- uugAGCGCgugAGUGCGGAGGGACaG- -5' |
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12460 | 3' | -58.7 | NC_003324.1 | + | 33751 | 0.73 | 0.1974 |
Target: 5'- aAACUCGcCGuCACagCACGUCUCCCaGUCa -3' miRNA: 3'- -UUGAGC-GC-GUGa-GUGCGGAGGGaCAG- -5' |
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12460 | 3' | -58.7 | NC_003324.1 | + | 5803 | 0.73 | 0.182259 |
Target: 5'- -uCUCGUGCAC-CGCGCCUgCCUGc- -3' miRNA: 3'- uuGAGCGCGUGaGUGCGGAgGGACag -5' |
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12460 | 3' | -58.7 | NC_003324.1 | + | 44235 | 0.74 | 0.159301 |
Target: 5'- --aUCGCGCGCaacgcugugCACGCUUUCCUGUCc -3' miRNA: 3'- uugAGCGCGUGa--------GUGCGGAGGGACAG- -5' |
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12460 | 3' | -58.7 | NC_003324.1 | + | 34112 | 1.08 | 0.000508 |
Target: 5'- gAACUCGCGCACUCACGCCUCCCUGUCg -3' miRNA: 3'- -UUGAGCGCGUGAGUGCGGAGGGACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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