Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12460 | 5' | -58.2 | NC_003324.1 | + | 34148 | 1.05 | 0.000937 |
Target: 5'- cAGGUCAGGGACCGGCACAAGCCGUAUc -3' miRNA: 3'- -UCCAGUCCCUGGCCGUGUUCGGCAUA- -5' |
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12460 | 5' | -58.2 | NC_003324.1 | + | 16551 | 0.71 | 0.274722 |
Target: 5'- aGGGUgAGGGGCUGGCugcuCAucgucucGCCGUAg -3' miRNA: 3'- -UCCAgUCCCUGGCCGu---GUu------CGGCAUa -5' |
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12460 | 5' | -58.2 | NC_003324.1 | + | 41187 | 0.71 | 0.261194 |
Target: 5'- cAGG--AGGGACC-GCAUGAGCCGUAg -3' miRNA: 3'- -UCCagUCCCUGGcCGUGUUCGGCAUa -5' |
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12460 | 5' | -58.2 | NC_003324.1 | + | 14571 | 0.7 | 0.296058 |
Target: 5'- uGGGUCGcGGccuGCCGGCGCAagcGGCCGg-- -3' miRNA: 3'- -UCCAGUcCC---UGGCCGUGU---UCGGCaua -5' |
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12460 | 5' | -58.2 | NC_003324.1 | + | 8414 | 0.7 | 0.317885 |
Target: 5'- cGGGUCAGGGauuuccGCCGGCugcagguGCAGGCgGa-- -3' miRNA: 3'- -UCCAGUCCC------UGGCCG-------UGUUCGgCaua -5' |
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12460 | 5' | -58.2 | NC_003324.1 | + | 54256 | 0.67 | 0.499383 |
Target: 5'- cGGUugcCAuGGACCGGCAUcagcgccGAGCCGUu- -3' miRNA: 3'- uCCA---GUcCCUGGCCGUG-------UUCGGCAua -5' |
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12460 | 5' | -58.2 | NC_003324.1 | + | 20326 | 0.67 | 0.50041 |
Target: 5'- cGGUCGGGGuucgccaucGCCGaCAgAAGCCGg-- -3' miRNA: 3'- uCCAGUCCC---------UGGCcGUgUUCGGCaua -5' |
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12460 | 5' | -58.2 | NC_003324.1 | + | 46660 | 0.66 | 0.531621 |
Target: 5'- aAGGUCugcguGGGAuaGGCugGaaAGCCGUc- -3' miRNA: 3'- -UCCAGu----CCCUggCCGugU--UCGGCAua -5' |
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12460 | 5' | -58.2 | NC_003324.1 | + | 37298 | 0.66 | 0.552814 |
Target: 5'- cGGUUgacuGGGuaAUCGGCACAgagaagcgcAGCCGUAc -3' miRNA: 3'- uCCAGu---CCC--UGGCCGUGU---------UCGGCAUa -5' |
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12460 | 5' | -58.2 | NC_003324.1 | + | 53669 | 0.66 | 0.542184 |
Target: 5'- gAGuGUCaugucgcaaagGGGGACCGGCAuCAGGCgGc-- -3' miRNA: 3'- -UC-CAG-----------UCCCUGGCCGU-GUUCGgCaua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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