miRNA display CGI


Results 1 - 16 of 16 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12462 5' -56.1 NC_003324.1 + 38069 1.08 0.000993
Target:  5'- cUCGCGGUACUGCGCCUUCUGGCGAAGc -3'
miRNA:   3'- -AGCGCCAUGACGCGGAAGACCGCUUC- -5'
12462 5' -56.1 NC_003324.1 + 22259 0.74 0.266386
Target:  5'- aUCGCGGcgaGCUGCGCCUUgaugucgucgUUGGUGAu- -3'
miRNA:   3'- -AGCGCCa--UGACGCGGAA----------GACCGCUuc -5'
12462 5' -56.1 NC_003324.1 + 31318 0.73 0.273217
Target:  5'- gUGCGGccgGCgGCGCUggcgCUGGCGggGg -3'
miRNA:   3'- aGCGCCa--UGaCGCGGaa--GACCGCuuC- -5'
12462 5' -56.1 NC_003324.1 + 29034 0.72 0.32499
Target:  5'- aCGCGGUACcGauagucaucCGCCaugCUGGCGAGGu -3'
miRNA:   3'- aGCGCCAUGaC---------GCGGaa-GACCGCUUC- -5'
12462 5' -56.1 NC_003324.1 + 23192 0.71 0.392655
Target:  5'- gCGCGGUACcgcucugcaucgUGCGCCagaugaagcucUUCUGGUGcGGg -3'
miRNA:   3'- aGCGCCAUG------------ACGCGG-----------AAGACCGCuUC- -5'
12462 5' -56.1 NC_003324.1 + 50440 0.7 0.410945
Target:  5'- gCGUGGUgcagcucgcaggGCUGCGCgaUCcggagGGCGAAGa -3'
miRNA:   3'- aGCGCCA------------UGACGCGgaAGa----CCGCUUC- -5'
12462 5' -56.1 NC_003324.1 + 24408 0.7 0.410945
Target:  5'- aUGCGGauaugcACUGCGCCgUCgGGCGAu- -3'
miRNA:   3'- aGCGCCa-----UGACGCGGaAGaCCGCUuc -5'
12462 5' -56.1 NC_003324.1 + 56349 0.68 0.551892
Target:  5'- aUCuCGGUcauuGCUGCGuccCCUUCuguUGGCGAAGc -3'
miRNA:   3'- -AGcGCCA----UGACGC---GGAAG---ACCGCUUC- -5'
12462 5' -56.1 NC_003324.1 + 56515 0.68 0.558326
Target:  5'- gCGCGGcGCUGCGCacaacaUUUggcaugcgaugcgGGCGAAGu -3'
miRNA:   3'- aGCGCCaUGACGCGg-----AAGa------------CCGCUUC- -5'
12462 5' -56.1 NC_003324.1 + 3624 0.68 0.573415
Target:  5'- gUGUGG-GCUGCauagcCCUUCuUGGCGggGg -3'
miRNA:   3'- aGCGCCaUGACGc----GGAAG-ACCGCuuC- -5'
12462 5' -56.1 NC_003324.1 + 2827 0.67 0.595127
Target:  5'- cUGCGGUAUUuuCGCCg---GGCGAGGg -3'
miRNA:   3'- aGCGCCAUGAc-GCGGaagaCCGCUUC- -5'
12462 5' -56.1 NC_003324.1 + 24077 0.67 0.638851
Target:  5'- cUCGcCGGUgcGCUGCGCCacggUGGCGcuGa -3'
miRNA:   3'- -AGC-GCCA--UGACGCGGaag-ACCGCuuC- -5'
12462 5' -56.1 NC_003324.1 + 25302 0.66 0.660715
Target:  5'- cUGCGGaggugUAgUGCGCgUUUCUGGUGGAc -3'
miRNA:   3'- aGCGCC-----AUgACGCG-GAAGACCGCUUc -5'
12462 5' -56.1 NC_003324.1 + 42928 0.66 0.682473
Target:  5'- -gGCGGUcACUGCGCC----GGCGGu- -3'
miRNA:   3'- agCGCCA-UGACGCGGaagaCCGCUuc -5'
12462 5' -56.1 NC_003324.1 + 53406 0.66 0.682473
Target:  5'- aUCGCGGUcACgGCGCCUaCUacGGCu--- -3'
miRNA:   3'- -AGCGCCA-UGaCGCGGAaGA--CCGcuuc -5'
12462 5' -56.1 NC_003324.1 + 3242 0.66 0.697596
Target:  5'- gCGCGGUGCcgGUGCUUUCggagcucgccaaggGGCGc-- -3'
miRNA:   3'- aGCGCCAUGa-CGCGGAAGa-------------CCGCuuc -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.