Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12463 | 3' | -55.6 | NC_003324.1 | + | 18305 | 0.67 | 0.654416 |
Target: 5'- -cGCUGCCccggccuUGGUGAGACCgccaaAGcgGGCGc -3' miRNA: 3'- gcUGGCGG-------ACCACUUUGG-----UCuaCCGC- -5' |
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12463 | 3' | -55.6 | NC_003324.1 | + | 20734 | 0.69 | 0.540642 |
Target: 5'- -cGCCGCCUGGUcgaagcacuccgcccGAAGCCAaagGGCa -3' miRNA: 3'- gcUGGCGGACCA---------------CUUUGGUcuaCCGc -5' |
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12463 | 3' | -55.6 | NC_003324.1 | + | 24025 | 0.66 | 0.709525 |
Target: 5'- gCGugCGCUUGGaGA----AGAUGGCGg -3' miRNA: 3'- -GCugGCGGACCaCUuuggUCUACCGC- -5' |
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12463 | 3' | -55.6 | NC_003324.1 | + | 27185 | 0.74 | 0.291663 |
Target: 5'- gCGACCGCgCUGGUGugcugcGCCAGAUG-Ca -3' miRNA: 3'- -GCUGGCG-GACCACuu----UGGUCUACcGc -5' |
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12463 | 3' | -55.6 | NC_003324.1 | + | 29471 | 0.67 | 0.677251 |
Target: 5'- aCGGCCGCCUGGcUGcGGGCgCGucgGGCGc -3' miRNA: 3'- -GCUGGCGGACC-AC-UUUG-GUcuaCCGC- -5' |
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12463 | 3' | -55.6 | NC_003324.1 | + | 31018 | 0.66 | 0.688066 |
Target: 5'- uCGAUCGCUUGGUaGcGACCGGcgcggucGGCGu -3' miRNA: 3'- -GCUGGCGGACCA-CuUUGGUCua-----CCGC- -5' |
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12463 | 3' | -55.6 | NC_003324.1 | + | 38490 | 1.1 | 0.000936 |
Target: 5'- cCGACCGCCUGGUGAAACCAGAUGGCGa -3' miRNA: 3'- -GCUGGCGGACCACUUUGGUCUACCGC- -5' |
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12463 | 3' | -55.6 | NC_003324.1 | + | 38737 | 0.66 | 0.709525 |
Target: 5'- aGGCgCGCUUGGccucUGAAAUCGGgcGGCc -3' miRNA: 3'- gCUG-GCGGACC----ACUUUGGUCuaCCGc -5' |
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12463 | 3' | -55.6 | NC_003324.1 | + | 39810 | 0.72 | 0.361821 |
Target: 5'- aGGCCGCCaugUGGUGGcugcagaaccgcaGGCCGGGcacaUGGCGc -3' miRNA: 3'- gCUGGCGG---ACCACU-------------UUGGUCU----ACCGC- -5' |
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12463 | 3' | -55.6 | NC_003324.1 | + | 44636 | 0.7 | 0.452622 |
Target: 5'- uGACUGCCcaugcgaugccgGGUGu--CCAGAUGGUGu -3' miRNA: 3'- gCUGGCGGa-----------CCACuuuGGUCUACCGC- -5' |
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12463 | 3' | -55.6 | NC_003324.1 | + | 49107 | 0.69 | 0.557704 |
Target: 5'- -cGCUGCC-GGUGAcgcgaucGCCaAGAUGGCGg -3' miRNA: 3'- gcUGGCGGaCCACUu------UGG-UCUACCGC- -5' |
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12463 | 3' | -55.6 | NC_003324.1 | + | 49148 | 0.67 | 0.666394 |
Target: 5'- uCGGCC-CC-GGUGcaGAGCCGGAUGGa- -3' miRNA: 3'- -GCUGGcGGaCCAC--UUUGGUCUACCgc -5' |
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12463 | 3' | -55.6 | NC_003324.1 | + | 54806 | 0.69 | 0.515394 |
Target: 5'- aGACCGCCgcgccguucgcGGUGAGACCGGcagGGa- -3' miRNA: 3'- gCUGGCGGa----------CCACUUUGGUCua-CCgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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