Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12464 | 3' | -54.4 | NC_003324.1 | + | 13282 | 0.66 | 0.758212 |
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Target: 5'- aGCAGGUgCUCuGAUgGCGUUcaACgAUCGGGa -3' miRNA: 3'- -UGUCCAaGAG-CUA-CGCGG--UG-UAGCCC- -5' |
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| 12464 | 3' | -54.4 | NC_003324.1 | + | 25731 | 0.66 | 0.757187 |
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Target: 5'- gACGGcGUgaacuucUCUCGGUGCGCCGCGa---- -3' miRNA: 3'- -UGUC-CA-------AGAGCUACGCGGUGUagccc -5' |
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| 12464 | 3' | -54.4 | NC_003324.1 | + | 12816 | 0.68 | 0.683965 |
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Target: 5'- cCAGcgUUUCGAcGCGCCGCAagGGGc -3' miRNA: 3'- uGUCcaAGAGCUaCGCGGUGUagCCC- -5' |
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| 12464 | 3' | -54.4 | NC_003324.1 | + | 51194 | 0.68 | 0.673071 |
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Target: 5'- -gGGGUUCUgaGAUGCGCgACAUCc-- -3' miRNA: 3'- ugUCCAAGAg-CUACGCGgUGUAGccc -5' |
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| 12464 | 3' | -54.4 | NC_003324.1 | + | 24246 | 0.68 | 0.651179 |
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Target: 5'- cGCAGGUUCUCGAucUGCugaucagagGCCuucuCGUCGa- -3' miRNA: 3'- -UGUCCAAGAGCU--ACG---------CGGu---GUAGCcc -5' |
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| 12464 | 3' | -54.4 | NC_003324.1 | + | 51769 | 0.69 | 0.618235 |
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Target: 5'- uGCAGGcgCUCGAcGCGa-ACGUCGGu -3' miRNA: 3'- -UGUCCaaGAGCUaCGCggUGUAGCCc -5' |
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| 12464 | 3' | -54.4 | NC_003324.1 | + | 29416 | 0.69 | 0.585398 |
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Target: 5'- cAUAGG-UCUCGAUGCG-CGC--CGGGa -3' miRNA: 3'- -UGUCCaAGAGCUACGCgGUGuaGCCC- -5' |
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| 12464 | 3' | -54.4 | NC_003324.1 | + | 48200 | 0.7 | 0.525198 |
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Target: 5'- cGCAGGUUguacuugaaguccgcUUCGAgcaGCaGCCACGUCGGc -3' miRNA: 3'- -UGUCCAA---------------GAGCUa--CG-CGGUGUAGCCc -5' |
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| 12464 | 3' | -54.4 | NC_003324.1 | + | 22094 | 0.76 | 0.259543 |
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Target: 5'- gGCAGGUUCUCGgcGCGCCAgAcgaUCGa- -3' miRNA: 3'- -UGUCCAAGAGCuaCGCGGUgU---AGCcc -5' |
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| 12464 | 3' | -54.4 | NC_003324.1 | + | 39452 | 1.1 | 0.001271 |
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Target: 5'- uACAGGUUCUCGAUGCGCCACAUCGGGu -3' miRNA: 3'- -UGUCCAAGAGCUACGCGGUGUAGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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