Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12466 | 5' | -50.6 | NC_003324.1 | + | 20493 | 0.7 | 0.820634 |
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Target: 5'- aCCGcUUGCGAUCgACGAugacggcuccguUGUCGAauACGGc -3' miRNA: 3'- aGGU-AGCGCUAG-UGCU------------ACAGCU--UGCC- -5' |
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| 12466 | 5' | -50.6 | NC_003324.1 | + | 2674 | 0.7 | 0.815012 |
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Target: 5'- uUCCAgCGCGguggccgcuucggcaAgggCACGAUGUCGGcgGCGGc -3' miRNA: 3'- -AGGUaGCGC---------------Ua--GUGCUACAGCU--UGCC- -5' |
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| 12466 | 5' | -50.6 | NC_003324.1 | + | 21503 | 0.72 | 0.675599 |
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Target: 5'- cCCGUCGCGuugcUCAag--GUCGGACGGc -3' miRNA: 3'- aGGUAGCGCu---AGUgcuaCAGCUUGCC- -5' |
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| 12466 | 5' | -50.6 | NC_003324.1 | + | 41393 | 0.75 | 0.543192 |
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Target: 5'- -aCAUCGUGAUCGCGAUGgaaaggcCGcACGGc -3' miRNA: 3'- agGUAGCGCUAGUGCUACa------GCuUGCC- -5' |
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| 12466 | 5' | -50.6 | NC_003324.1 | + | 19207 | 0.76 | 0.469626 |
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Target: 5'- cUCCAUCGCcuuGcgCACGuUGUCGAGCaGGa -3' miRNA: 3'- -AGGUAGCG---CuaGUGCuACAGCUUG-CC- -5' |
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| 12466 | 5' | -50.6 | NC_003324.1 | + | 32490 | 0.76 | 0.459523 |
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Target: 5'- gCC-UCgGCGAUCACGGUGUCG-ACGa -3' miRNA: 3'- aGGuAG-CGCUAGUGCUACAGCuUGCc -5' |
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| 12466 | 5' | -50.6 | NC_003324.1 | + | 18341 | 0.78 | 0.40149 |
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Target: 5'- uUCCGUCaGCGGUCGCGGcucUGUCGucGAUGGc -3' miRNA: 3'- -AGGUAG-CGCUAGUGCU---ACAGC--UUGCC- -5' |
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| 12466 | 5' | -50.6 | NC_003324.1 | + | 41317 | 1.11 | 0.003017 |
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Target: 5'- uUCCAUCGCGAUCACGAUGUCGAACGGc -3' miRNA: 3'- -AGGUAGCGCUAGUGCUACAGCUUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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