Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12467 | 3' | -61.2 | NC_003324.1 | + | 42392 | 0.67 | 0.392388 |
Target: 5'- uGUCACcGGCGCcCGgcaCGUGGCaGGCGg -3' miRNA: 3'- -CGGUGcCCGCGaGCa--GCACCGcUCGU- -5' |
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12467 | 3' | -61.2 | NC_003324.1 | + | 22695 | 0.67 | 0.366982 |
Target: 5'- cGUCGCGGG-GCUCGgcaaCGcGGCaGGCAg -3' miRNA: 3'- -CGGUGCCCgCGAGCa---GCaCCGcUCGU- -5' |
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12467 | 3' | -61.2 | NC_003324.1 | + | 57266 | 0.7 | 0.251257 |
Target: 5'- aCCAC-GGCGCUUGUCuGUGaGCG-GCAg -3' miRNA: 3'- cGGUGcCCGCGAGCAG-CAC-CGCuCGU- -5' |
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12467 | 3' | -61.2 | NC_003324.1 | + | 31624 | 0.74 | 0.139198 |
Target: 5'- aCCGCGGGCGCg---CGUGGCG-GCc -3' miRNA: 3'- cGGUGCCCGCGagcaGCACCGCuCGu -5' |
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12467 | 3' | -61.2 | NC_003324.1 | + | 20606 | 0.74 | 0.118611 |
Target: 5'- cGCUgAUGGGCGCUUG-CGgucGGCGGGCAc -3' miRNA: 3'- -CGG-UGCCCGCGAGCaGCa--CCGCUCGU- -5' |
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12467 | 3' | -61.2 | NC_003324.1 | + | 41486 | 1.1 | 0.000262 |
Target: 5'- aGCCACGGGCGCUCGUCGUGGCGAGCAa -3' miRNA: 3'- -CGGUGCCCGCGAGCAGCACCGCUCGU- -5' |
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12467 | 3' | -61.2 | NC_003324.1 | + | 50424 | 0.69 | 0.270416 |
Target: 5'- uGCCGCaaGGCGCUgGgCGUGGUGcAGCu -3' miRNA: 3'- -CGGUGc-CCGCGAgCaGCACCGC-UCGu -5' |
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12467 | 3' | -61.2 | NC_003324.1 | + | 21089 | 0.68 | 0.307835 |
Target: 5'- cGCCGCGGcGCGUgcugCGUCGgcuuccacgucgggcUGGCGcuuGCGc -3' miRNA: 3'- -CGGUGCC-CGCGa---GCAGC---------------ACCGCu--CGU- -5' |
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12467 | 3' | -61.2 | NC_003324.1 | + | 41636 | 0.67 | 0.366982 |
Target: 5'- uGCCACGGcucgccCGCUC-UCGUcGGCGGcGCGa -3' miRNA: 3'- -CGGUGCCc-----GCGAGcAGCA-CCGCU-CGU- -5' |
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12467 | 3' | -61.2 | NC_003324.1 | + | 28637 | 0.67 | 0.383793 |
Target: 5'- cGUUGCGGGCcgccuugucGCgCGUCGUGGUGccaucGGCAu -3' miRNA: 3'- -CGGUGCCCG---------CGaGCAGCACCGC-----UCGU- -5' |
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12467 | 3' | -61.2 | NC_003324.1 | + | 15656 | 0.67 | 0.392388 |
Target: 5'- gGCCcCGGcGCGCUCGaugacgCGaucaaGGCGGGCc -3' miRNA: 3'- -CGGuGCC-CGCGAGCa-----GCa----CCGCUCGu -5' |
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12467 | 3' | -61.2 | NC_003324.1 | + | 27060 | 0.66 | 0.409949 |
Target: 5'- cGCgACGcGGCGUUacCGUUGUGGCcGGUg -3' miRNA: 3'- -CGgUGC-CCGCGA--GCAGCACCGcUCGu -5' |
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12467 | 3' | -61.2 | NC_003324.1 | + | 29481 | 0.66 | 0.42799 |
Target: 5'- gGCUGCGGGCGC--GUCG-GGCGcaucucGGUAa -3' miRNA: 3'- -CGGUGCCCGCGagCAGCaCCGC------UCGU- -5' |
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12467 | 3' | -61.2 | NC_003324.1 | + | 23944 | 0.66 | 0.436261 |
Target: 5'- uUCACGcGGCGaagUCGUUcaagacgGUGGCGAGUg -3' miRNA: 3'- cGGUGC-CCGCg--AGCAG-------CACCGCUCGu -5' |
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12467 | 3' | -61.2 | NC_003324.1 | + | 8325 | 0.66 | 0.437185 |
Target: 5'- cGCCGucGGCGCUCGUuacggugacCGUcGGCGGGgAg -3' miRNA: 3'- -CGGUgcCCGCGAGCA---------GCA-CCGCUCgU- -5' |
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12467 | 3' | -61.2 | NC_003324.1 | + | 50189 | 0.66 | 0.45591 |
Target: 5'- cGCCAUGGGCaucaGCgacaaGUC--GGCGAGCc -3' miRNA: 3'- -CGGUGCCCG----CGag---CAGcaCCGCUCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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