Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12467 | 5' | -52.7 | NC_003324.1 | + | 25860 | 0.66 | 0.798914 |
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Target: 5'- cGGUACC-GCCAagACGugGC-UGCUGAc -3' miRNA: 3'- -CCAUGGaCGGU--UGUugCGcACGGUUu -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 3278 | 0.66 | 0.807774 |
|
Target: 5'- aGG-GCCUGCCAcucaucaaaGCAaucccauucaucgGCGCGgUGCCGGu -3' miRNA: 3'- -CCaUGGACGGU---------UGU-------------UGCGC-ACGGUUu -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 29989 | 0.66 | 0.837032 |
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Target: 5'- --cGCCguccagcaugGCCAGCAGCGC--GCCGAAg -3' miRNA: 3'- ccaUGGa---------CGGUUGUUGCGcaCGGUUU- -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 29101 | 0.66 | 0.837032 |
|
Target: 5'- cGGUACCgcGUCGA--GCGCGcUGCUAAGg -3' miRNA: 3'- -CCAUGGa-CGGUUguUGCGC-ACGGUUU- -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 39261 | 0.66 | 0.846017 |
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Target: 5'- cGGUugCcGCCGugGcuGCGCcagaagGUGCCGAu -3' miRNA: 3'- -CCAugGaCGGUugU--UGCG------CACGGUUu -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 12755 | 0.69 | 0.658712 |
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Target: 5'- --aAUCgaGCCAGCGAUGCGUucGCCAGAc -3' miRNA: 3'- ccaUGGa-CGGUUGUUGCGCA--CGGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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