Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12470 | 5' | -54.2 | NC_003324.1 | + | 42323 | 1.09 | 0.001363 |
Target: 5'- gGCGCCGGUGACAGACUUAGAGCGACUu -3' miRNA: 3'- -CGCGGCCACUGUCUGAAUCUCGCUGA- -5' |
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12470 | 5' | -54.2 | NC_003324.1 | + | 7775 | 0.73 | 0.406436 |
Target: 5'- cGCGCCGGUGuuuccggggucgGCGGugUgc-AGCGACa -3' miRNA: 3'- -CGCGGCCAC------------UGUCugAaucUCGCUGa -5' |
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12470 | 5' | -54.2 | NC_003324.1 | + | 55029 | 0.7 | 0.548072 |
Target: 5'- cGCGCCGGgcaggcaaGGCGGACggcUGGGcugccGCGACg -3' miRNA: 3'- -CGCGGCCa-------CUGUCUGa--AUCU-----CGCUGa -5' |
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12470 | 5' | -54.2 | NC_003324.1 | + | 12522 | 0.69 | 0.591642 |
Target: 5'- cGgGCUGGUGGCAGuucgAG-GCGACg -3' miRNA: 3'- -CgCGGCCACUGUCugaaUCuCGCUGa -5' |
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12470 | 5' | -54.2 | NC_003324.1 | + | 10809 | 0.69 | 0.602641 |
Target: 5'- gGCGCUGGUGgu-GGCUgcacGAGUGACg -3' miRNA: 3'- -CGCGGCCACuguCUGAau--CUCGCUGa -5' |
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12470 | 5' | -54.2 | NC_003324.1 | + | 51532 | 0.67 | 0.712407 |
Target: 5'- -gGCCGGUGGCuuGGGCUgccgucuuGCGGCa -3' miRNA: 3'- cgCGGCCACUG--UCUGAaucu----CGCUGa -5' |
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12470 | 5' | -54.2 | NC_003324.1 | + | 31100 | 0.67 | 0.73376 |
Target: 5'- cGCGCCGGUcgcuaccaagcGAUcGACg-AGAGCGaACUg -3' miRNA: 3'- -CGCGGCCA-----------CUGuCUGaaUCUCGC-UGA- -5' |
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12470 | 5' | -54.2 | NC_003324.1 | + | 40060 | 0.67 | 0.701606 |
Target: 5'- aCGCuCGGcgcGACGGACUUGGAGauugcgcaGGCUu -3' miRNA: 3'- cGCG-GCCa--CUGUCUGAAUCUCg-------CUGA- -5' |
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12470 | 5' | -54.2 | NC_003324.1 | + | 39839 | 0.66 | 0.754702 |
Target: 5'- -aGCCGGUGGCAcGCUUGuAGaGGCUg -3' miRNA: 3'- cgCGGCCACUGUcUGAAUcUCgCUGA- -5' |
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12470 | 5' | -54.2 | NC_003324.1 | + | 23996 | 0.66 | 0.764989 |
Target: 5'- cGCGCUGGUagGGCGGcggUAGGGCuGCUg -3' miRNA: 3'- -CGCGGCCA--CUGUCugaAUCUCGcUGA- -5' |
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12470 | 5' | -54.2 | NC_003324.1 | + | 32747 | 0.66 | 0.794981 |
Target: 5'- cCGCCgaccgguuuGGUGGCGGGCUUu--GUGACg -3' miRNA: 3'- cGCGG---------CCACUGUCUGAAucuCGCUGa -5' |
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12470 | 5' | -54.2 | NC_003324.1 | + | 14809 | 0.66 | 0.804651 |
Target: 5'- cGCgGCCGGcaucGGCAG-CUUccuGGGCGGCa -3' miRNA: 3'- -CG-CGGCCa---CUGUCuGAAu--CUCGCUGa -5' |
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12470 | 5' | -54.2 | NC_003324.1 | + | 35228 | 0.66 | 0.804651 |
Target: 5'- gGCGCCgaaGGUGACgaAGGCgcugAGgucaacGGCGACg -3' miRNA: 3'- -CGCGG---CCACUG--UCUGaa--UC------UCGCUGa -5' |
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12470 | 5' | -54.2 | NC_003324.1 | + | 41812 | 0.66 | 0.794981 |
Target: 5'- gGUGCC-GUGGCGuGAUcgcaUGGAGCGACc -3' miRNA: 3'- -CGCGGcCACUGU-CUGa---AUCUCGCUGa -5' |
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12470 | 5' | -54.2 | NC_003324.1 | + | 27180 | 0.66 | 0.754702 |
Target: 5'- cGCGCUGGUGugcugcgcCAGAUgcagGGAGCcGCa -3' miRNA: 3'- -CGCGGCCACu-------GUCUGaa--UCUCGcUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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