Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12471 | 5' | -55.5 | NC_003324.1 | + | 31235 | 0.66 | 0.653853 |
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Target: 5'- cGCCAgcGCCAG-CGccGCcgGCCGCAc -3' miRNA: 3'- -UGGUaaCGGUCaGCa-CGaaCGGCGUu -5' |
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| 12471 | 5' | -55.5 | NC_003324.1 | + | 32518 | 0.66 | 0.649359 |
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Target: 5'- uGCCGUUGCCAGcgucaucaagaacCGUGCUggUGUgCGCGGu -3' miRNA: 3'- -UGGUAACGGUCa------------GCACGA--ACG-GCGUU- -5' |
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| 12471 | 5' | -55.5 | NC_003324.1 | + | 14881 | 0.67 | 0.586481 |
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Target: 5'- uGCCGaUGCCGGccgCGUuCUUcGCCGCGAu -3' miRNA: 3'- -UGGUaACGGUCa--GCAcGAA-CGGCGUU- -5' |
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| 12471 | 5' | -55.5 | NC_003324.1 | + | 20832 | 0.67 | 0.575335 |
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Target: 5'- cGCCGUU-CCGGUCGgguccaUGUcgGCCGCAu -3' miRNA: 3'- -UGGUAAcGGUCAGC------ACGaaCGGCGUu -5' |
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| 12471 | 5' | -55.5 | NC_003324.1 | + | 33914 | 0.68 | 0.541133 |
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Target: 5'- uACgAUUGCCgcgaggcGGcCGUGCaaGCCGCAAu -3' miRNA: 3'- -UGgUAACGG-------UCaGCACGaaCGGCGUU- -5' |
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| 12471 | 5' | -55.5 | NC_003324.1 | + | 11280 | 0.68 | 0.509773 |
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Target: 5'- gGCCAUguacugauuuuUGCCGGUCGUGgUcauCCGCAc -3' miRNA: 3'- -UGGUA-----------ACGGUCAGCACgAac-GGCGUu -5' |
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| 12471 | 5' | -55.5 | NC_003324.1 | + | 56787 | 0.69 | 0.478161 |
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Target: 5'- aGCCGguaagcgUGCCGgaggguGUCGaGCUUGCCGCu- -3' miRNA: 3'- -UGGUa------ACGGU------CAGCaCGAACGGCGuu -5' |
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| 12471 | 5' | -55.5 | NC_003324.1 | + | 42547 | 1.05 | 0.001269 |
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Target: 5'- cACCAUUGCCAGUCGUGCUUGCCGCAAu -3' miRNA: 3'- -UGGUAACGGUCAGCACGAACGGCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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