Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12472 | 3' | -57.4 | NC_003324.1 | + | 41555 | 0.66 | 0.556798 |
Target: 5'- aCGACAGCGgcgcGGGCCuguuccUCGCagGuGGCCGu -3' miRNA: 3'- -GUUGUCGU----UCCGGua----AGCGg-C-CCGGU- -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 32112 | 0.66 | 0.586027 |
Target: 5'- --cCGGUGAGGCCGagUGCCuuggucuccgccucGGGCCGg -3' miRNA: 3'- guuGUCGUUCCGGUaaGCGG--------------CCCGGU- -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 55035 | 0.66 | 0.600209 |
Target: 5'- gGGCAgGCAAGGCgGacgGCUGGGCUg -3' miRNA: 3'- gUUGU-CGUUCCGgUaagCGGCCCGGu -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 4693 | 0.66 | 0.600209 |
Target: 5'- uCAGCGGCAucauguuGGCguUcuUCGgaucCCGGGCCGa -3' miRNA: 3'- -GUUGUCGUu------CCGguA--AGC----GGCCCGGU- -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 31322 | 0.66 | 0.589294 |
Target: 5'- -cGCAGUgcGGCCGgcggCGCUGGcGCUg -3' miRNA: 3'- guUGUCGuuCCGGUaa--GCGGCC-CGGu -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 3162 | 0.66 | 0.589294 |
Target: 5'- gAAUGGCAAGaGCgGgagCGCCGugcGGCCAu -3' miRNA: 3'- gUUGUCGUUC-CGgUaa-GCGGC---CCGGU- -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 14513 | 0.66 | 0.546075 |
Target: 5'- --cCGGCAuGGGCCcagaGCCGGGCg- -3' miRNA: 3'- guuGUCGU-UCCGGuaagCGGCCCGgu -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 3321 | 0.67 | 0.535419 |
Target: 5'- gGAUuGCAGGGCUuagucgCGCUGGGCg- -3' miRNA: 3'- gUUGuCGUUCCGGuaa---GCGGCCCGgu -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 11643 | 0.67 | 0.524837 |
Target: 5'- gAGCAGCAucGGCUGcUCGCCcgcaaaGGCCGa -3' miRNA: 3'- gUUGUCGUu-CCGGUaAGCGGc-----CCGGU- -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 17256 | 0.67 | 0.493597 |
Target: 5'- cCGACAGaCGcgccAGcGCCucgucaaUCGCUGGGCCAu -3' miRNA: 3'- -GUUGUC-GU----UC-CGGua-----AGCGGCCCGGU- -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 32454 | 0.67 | 0.523783 |
Target: 5'- gCAACGGCA--GCCAUgcccucgucgccuUCGCCGGcaGCCu -3' miRNA: 3'- -GUUGUCGUucCGGUA-------------AGCGGCC--CGGu -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 30665 | 0.67 | 0.483373 |
Target: 5'- aGAUAGCugcgcggaucGcGCCA--CGCCGGGCCAa -3' miRNA: 3'- gUUGUCGuu--------C-CGGUaaGCGGCCCGGU- -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 41128 | 0.67 | 0.483373 |
Target: 5'- -cGCuGCAAGGCUA-UCGCCGGcUCAc -3' miRNA: 3'- guUGuCGUUCCGGUaAGCGGCCcGGU- -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 6264 | 0.67 | 0.518526 |
Target: 5'- -cGCAGCAAGGgCugccucucgcaugUCGUCGGGCa- -3' miRNA: 3'- guUGUCGUUCCgGua-----------AGCGGCCCGgu -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 35038 | 0.67 | 0.483373 |
Target: 5'- gCGGCAGCAGcGGCCugaucgUCGUCGcuGCCGg -3' miRNA: 3'- -GUUGUCGUU-CCGGua----AGCGGCc-CGGU- -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 9631 | 0.68 | 0.473251 |
Target: 5'- uGugAGCuGGGCCAacacCGCCGGcaacGCCAa -3' miRNA: 3'- gUugUCGuUCCGGUaa--GCGGCC----CGGU- -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 54405 | 0.68 | 0.432924 |
Target: 5'- gAACGGCAacuccccGGGaCCGc-UGCCGGGCCu -3' miRNA: 3'- gUUGUCGU-------UCC-GGUaaGCGGCCCGGu -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 614 | 0.68 | 0.473251 |
Target: 5'- gGACAuCAAGGCCuggcaGCUGGGCUu -3' miRNA: 3'- gUUGUcGUUCCGGuaag-CGGCCCGGu -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 36170 | 0.68 | 0.424341 |
Target: 5'- uCAGgAGCAGGGCuCAcUCgccacaGCCGGGCUc -3' miRNA: 3'- -GUUgUCGUUCCG-GUaAG------CGGCCCGGu -5' |
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12472 | 3' | -57.4 | NC_003324.1 | + | 51719 | 0.69 | 0.37858 |
Target: 5'- --uCAGCGAGGCCGacgcaaUCGCCgagGGcGCCAa -3' miRNA: 3'- guuGUCGUUCCGGUa-----AGCGG---CC-CGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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