Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12474 | 5' | -54.5 | NC_003324.1 | + | 43085 | 1.09 | 0.00131 |
Target: 5'- cCGAGAAUGUCGUCUCGCCAUCCGCCAg -3' miRNA: 3'- -GCUCUUACAGCAGAGCGGUAGGCGGU- -5' |
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12474 | 5' | -54.5 | NC_003324.1 | + | 23642 | 0.69 | 0.574518 |
Target: 5'- uGuGGAUGUacCGUCgccgagcgCGCCcgCCGCCAc -3' miRNA: 3'- gCuCUUACA--GCAGa-------GCGGuaGGCGGU- -5' |
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12474 | 5' | -54.5 | NC_003324.1 | + | 22137 | 0.67 | 0.716305 |
Target: 5'- uGAGAuUGcCG--UCGUCGUCCGCCu -3' miRNA: 3'- gCUCUuACaGCagAGCGGUAGGCGGu -5' |
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12474 | 5' | -54.5 | NC_003324.1 | + | 20317 | 0.67 | 0.73747 |
Target: 5'- aCGAGcuuccgGUCGgggUUCGCCAU-CGCCGa -3' miRNA: 3'- -GCUCuua---CAGCa--GAGCGGUAgGCGGU- -5' |
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12474 | 5' | -54.5 | NC_003324.1 | + | 26988 | 0.66 | 0.7479 |
Target: 5'- uGAGggUGUUG-C-CGCCGgucUCUGCCc -3' miRNA: 3'- gCUCuuACAGCaGaGCGGU---AGGCGGu -5' |
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12474 | 5' | -54.5 | NC_003324.1 | + | 44050 | 0.66 | 0.758212 |
Target: 5'- uCGAGGAUGgCcUCUUGCucCAUCCGCUc -3' miRNA: 3'- -GCUCUUACaGcAGAGCG--GUAGGCGGu -5' |
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12474 | 5' | -54.5 | NC_003324.1 | + | 57295 | 0.66 | 0.768397 |
Target: 5'- cCGGucGUGcCGccCUCGCC-UCCGCCGa -3' miRNA: 3'- -GCUcuUACaGCa-GAGCGGuAGGCGGU- -5' |
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12474 | 5' | -54.5 | NC_003324.1 | + | 4251 | 0.66 | 0.777443 |
Target: 5'- aCGAGGugaagGUCGaUCagaugCGCCAcggcguuUCCGCCGg -3' miRNA: 3'- -GCUCUua---CAGC-AGa----GCGGU-------AGGCGGU- -5' |
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12474 | 5' | -54.5 | NC_003324.1 | + | 43703 | 0.66 | 0.788335 |
Target: 5'- --cGAAUGUCGUCcgguacCGCCugCUGCCAa -3' miRNA: 3'- gcuCUUACAGCAGa-----GCGGuaGGCGGU- -5' |
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12474 | 5' | -54.5 | NC_003324.1 | + | 27753 | 0.66 | 0.798066 |
Target: 5'- aGGGcucUGUCGUCUUGCCcgaacCCaGCCAc -3' miRNA: 3'- gCUCuu-ACAGCAGAGCGGua---GG-CGGU- -5' |
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12474 | 5' | -54.5 | NC_003324.1 | + | 2626 | 0.66 | 0.778441 |
Target: 5'- -cGGcGUGcCGUCU-GCCGUCCGUCGc -3' miRNA: 3'- gcUCuUACaGCAGAgCGGUAGGCGGU- -5' |
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12474 | 5' | -54.5 | NC_003324.1 | + | 47645 | 0.66 | 0.768397 |
Target: 5'- gCGAGggUcGaUCGgccCUCGUCAUCCaCCAu -3' miRNA: 3'- -GCUCuuA-C-AGCa--GAGCGGUAGGcGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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