Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12475 | 3' | -49.3 | NC_003324.1 | + | 28220 | 0.66 | 0.968991 |
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Target: 5'- aAGGUC-GGCcgCAUCCGcCuuggcGGGUUGGa -3' miRNA: 3'- -UCUAGuUCGa-GUAGGCuGu----CCUAACC- -5' |
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| 12475 | 3' | -49.3 | NC_003324.1 | + | 54790 | 0.67 | 0.944009 |
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Target: 5'- cGAUCGAGC--GUUCGACcGGAuUUGGc -3' miRNA: 3'- uCUAGUUCGagUAGGCUGuCCU-AACC- -5' |
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| 12475 | 3' | -49.3 | NC_003324.1 | + | 24510 | 0.68 | 0.894392 |
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Target: 5'- ---cCGGGCUCGaccaugacauuuUCCGGCAGGGUccGGa -3' miRNA: 3'- ucuaGUUCGAGU------------AGGCUGUCCUAa-CC- -5' |
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| 12475 | 3' | -49.3 | NC_003324.1 | + | 21565 | 0.69 | 0.870994 |
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Target: 5'- cGA-CGGGCgcggCAUCCGGCAGGGc--- -3' miRNA: 3'- uCUaGUUCGa---GUAGGCUGUCCUaacc -5' |
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| 12475 | 3' | -49.3 | NC_003324.1 | + | 23335 | 0.7 | 0.826774 |
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Target: 5'- uAGAUCcugacGAGCUCGUCgCGGCGGGcgucAUUGu -3' miRNA: 3'- -UCUAG-----UUCGAGUAG-GCUGUCC----UAACc -5' |
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| 12475 | 3' | -49.3 | NC_003324.1 | + | 44941 | 1.11 | 0.003286 |
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Target: 5'- uAGAUCAAGCUCAUCCGACAGGAUUGGu -3' miRNA: 3'- -UCUAGUUCGAGUAGGCUGUCCUAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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