Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12478 | 3' | -47.8 | NC_003324.1 | + | 31697 | 0.66 | 0.986994 |
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Target: 5'- -cGAGUGGGu--GCAUgCCAACgccagCCg -3' miRNA: 3'- gaCUUACCCcuuCGUAaGGUUGaa---GG- -5' |
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| 12478 | 3' | -47.8 | NC_003324.1 | + | 31468 | 0.66 | 0.986994 |
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Target: 5'- uCUGGAUGGGGAaggacggcccgaAGCAgUUCAAUg--- -3' miRNA: 3'- -GACUUACCCCU------------UCGUaAGGUUGaagg -5' |
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| 12478 | 3' | -47.8 | NC_003324.1 | + | 35477 | 0.66 | 0.983039 |
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Target: 5'- -cGAgcGUGGGGAcGGCGaucgCCGuaccggcuGCUUCCa -3' miRNA: 3'- gaCU--UACCCCU-UCGUaa--GGU--------UGAAGG- -5' |
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| 12478 | 3' | -47.8 | NC_003324.1 | + | 47873 | 0.66 | 0.98075 |
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Target: 5'- -cGGcgGGGGAAGUuggaauGCUUCCc -3' miRNA: 3'- gaCUuaCCCCUUCGuaagguUGAAGG- -5' |
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| 12478 | 3' | -47.8 | NC_003324.1 | + | 29281 | 0.67 | 0.975491 |
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Target: 5'- -cGAucGGcGGAuuGCGUUCCAGCU-CCu -3' miRNA: 3'- gaCUuaCC-CCUu-CGUAAGGUUGAaGG- -5' |
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| 12478 | 3' | -47.8 | NC_003324.1 | + | 42416 | 0.67 | 0.975491 |
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Target: 5'- -gGAAUGGGGAAGuCGcUCUAAg-UCUg -3' miRNA: 3'- gaCUUACCCCUUC-GUaAGGUUgaAGG- -5' |
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| 12478 | 3' | -47.8 | NC_003324.1 | + | 47784 | 1.13 | 0.003473 |
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Target: 5'- cCUGAAUGGGGAAGCAUUCCAACUUCCc -3' miRNA: 3'- -GACUUACCCCUUCGUAAGGUUGAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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