Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12479 | 5' | -53.3 | NC_003324.1 | + | 50594 | 0.66 | 0.800545 |
Target: 5'- cUGGcUGCGUGCCAgcgcuguuUCAaAAGCugGGugAUCu -3' miRNA: 3'- -ACC-ACGCGCGGU--------AGU-UUCG--UCugUAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 51238 | 0.66 | 0.759618 |
Target: 5'- aUGGUcCGCGCCcuuAUCGAAggacgcaagacGCAGACAc- -3' miRNA: 3'- -ACCAcGCGCGG---UAGUUU-----------CGUCUGUag -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 55503 | 0.66 | 0.810341 |
Target: 5'- cGGUccacaCGaCGCCAcuguUCAAGGCGGGCuUCg -3' miRNA: 3'- aCCAc----GC-GCGGU----AGUUUCGUCUGuAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 5552 | 0.67 | 0.749011 |
Target: 5'- cGGuUGC-CGCCGcaaccuUCGccGCGGGCGUCa -3' miRNA: 3'- aCC-ACGcGCGGU------AGUuuCGUCUGUAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 17805 | 0.67 | 0.749011 |
Target: 5'- cUGGUaGCGUGCC-UCAcGGCGcGCAUUc -3' miRNA: 3'- -ACCA-CGCGCGGuAGUuUCGUcUGUAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 29708 | 0.67 | 0.738278 |
Target: 5'- cGGcGuUGCGCCA--GAAGCAGGCAg- -3' miRNA: 3'- aCCaC-GCGCGGUagUUUCGUCUGUag -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 44419 | 0.67 | 0.738278 |
Target: 5'- aGGgcgcagcGCGCGCCGUgGcuGCAGAggcUAUCg -3' miRNA: 3'- aCCa------CGCGCGGUAgUuuCGUCU---GUAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 3212 | 0.67 | 0.716486 |
Target: 5'- aGGgGCGCGUCgAUCAcgccuuugAGGCAGGCGc- -3' miRNA: 3'- aCCaCGCGCGG-UAGU--------UUCGUCUGUag -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 17886 | 0.68 | 0.653904 |
Target: 5'- --aUGCGCGCCGU-GAGGCAcgcuaccagaucgccGGCAUCa -3' miRNA: 3'- accACGCGCGGUAgUUUCGU---------------CUGUAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 35695 | 0.68 | 0.649384 |
Target: 5'- aUGcUGCGCGCagcaGAAGCcGACAUCg -3' miRNA: 3'- -ACcACGCGCGguagUUUCGuCUGUAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 15655 | 0.68 | 0.694341 |
Target: 5'- cGGccccgGCGCGCUcgaugacgcgAUCAAGGCGGGCc-- -3' miRNA: 3'- aCCa----CGCGCGG----------UAGUUUCGUCUGuag -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 23324 | 0.68 | 0.687644 |
Target: 5'- aGGUGuCGUuguagauccugacgaGCuCGUCGcGGCGGGCGUCa -3' miRNA: 3'- aCCAC-GCG---------------CG-GUAGUuUCGUCUGUAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 11850 | 0.68 | 0.683167 |
Target: 5'- aGGUGcCGCGCCcaacCGGGGCAuuGACAg- -3' miRNA: 3'- aCCAC-GCGCGGua--GUUUCGU--CUGUag -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 50374 | 0.69 | 0.626759 |
Target: 5'- aUGGUGaaCGCcuucuuGCCGUCGAccGGCGGcGCAUCa -3' miRNA: 3'- -ACCAC--GCG------CGGUAGUU--UCGUC-UGUAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 8269 | 0.69 | 0.626759 |
Target: 5'- aGGcGCGCGCCG-CcGGGCAGugAg- -3' miRNA: 3'- aCCaCGCGCGGUaGuUUCGUCugUag -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 38699 | 0.69 | 0.615449 |
Target: 5'- cGGUagaGgGCGUCAUCcucgccAAGCAGAUGUCa -3' miRNA: 3'- aCCA---CgCGCGGUAGu-----UUCGUCUGUAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 54314 | 0.69 | 0.638074 |
Target: 5'- cGGUGcCGCGCCuUCGcuuuGAGCAGcCGcUCu -3' miRNA: 3'- aCCAC-GCGCGGuAGU----UUCGUCuGU-AG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 48091 | 0.69 | 0.625628 |
Target: 5'- aGGauCGCGCCGacguggcugcugcUCGAAGCGGACuUCa -3' miRNA: 3'- aCCacGCGCGGU-------------AGUUUCGUCUGuAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 14707 | 0.69 | 0.638074 |
Target: 5'- aGGUGCGaugcaggGCUAUCGAGGCauGGGC-UCg -3' miRNA: 3'- aCCACGCg------CGGUAGUUUCG--UCUGuAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 5485 | 0.7 | 0.537241 |
Target: 5'- aGGUGCggGCGCCAUCuuccGAGCcGAaAUCg -3' miRNA: 3'- aCCACG--CGCGGUAGu---UUCGuCUgUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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