Results 21 - 30 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12479 | 5' | -53.3 | NC_003324.1 | + | 11498 | 0.71 | 0.515469 |
Target: 5'- aGGUGCGCGUuagggucgauugCGUCGAAGguGuugagaacacuuACAUCa -3' miRNA: 3'- aCCACGCGCG------------GUAGUUUCguC------------UGUAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 43268 | 0.71 | 0.494063 |
Target: 5'- gGGUGCGCcuuGCCuucGUCGAGGCuGGC-UCg -3' miRNA: 3'- aCCACGCG---CGG---UAGUUUCGuCUGuAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 582 | 0.71 | 0.483512 |
Target: 5'- cUGG-GCGCGaacugcguCCGUuggacCGAGGCGGACAUCa -3' miRNA: 3'- -ACCaCGCGC--------GGUA-----GUUUCGUCUGUAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 6507 | 0.72 | 0.451525 |
Target: 5'- cUGGUGCGUGCCcuucacgagcuggAUCGcuGCAGACu-- -3' miRNA: 3'- -ACCACGCGCGG-------------UAGUuuCGUCUGuag -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 51878 | 0.72 | 0.419758 |
Target: 5'- gGGUGgucgccuacaccuuCGCGUCAUC-AAGCAGaACAUCg -3' miRNA: 3'- aCCAC--------------GCGCGGUAGuUUCGUC-UGUAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 51437 | 0.73 | 0.38385 |
Target: 5'- gGGUGCGCGagaacgcugcuguCCGUCAccaGAGUuGGCAUCa -3' miRNA: 3'- aCCACGCGC-------------GGUAGU---UUCGuCUGUAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 30440 | 0.75 | 0.308084 |
Target: 5'- aGG-GCGaCGCCAUCAAGGCucuGACGg- -3' miRNA: 3'- aCCaCGC-GCGGUAGUUUCGu--CUGUag -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 23278 | 0.77 | 0.212919 |
Target: 5'- aGGcucagaUGCGCGCCGUCAuAGCugcgcaGGACAUCc -3' miRNA: 3'- aCC------ACGCGCGGUAGUuUCG------UCUGUAG- -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 55023 | 0.8 | 0.14414 |
Target: 5'- aGGUGuCGCGCCGggcaggCAAGGCGGACGg- -3' miRNA: 3'- aCCAC-GCGCGGUa-----GUUUCGUCUGUag -5' |
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12479 | 5' | -53.3 | NC_003324.1 | + | 48461 | 1.11 | 0.000972 |
Target: 5'- aUGGUGCGCGCCAUCAAAGCAGACAUCg -3' miRNA: 3'- -ACCACGCGCGGUAGUUUCGUCUGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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