Results 21 - 34 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12480 | 5' | -54.7 | NC_003324.1 | + | 18670 | 0.66 | 0.770082 |
Target: 5'- uGCCcuugCGGUggauGAAGCGCUUgcgugcggaAAGGUCGg -3' miRNA: 3'- cCGGua--GUCG----CUUCGCGAG---------UUCCAGC- -5' |
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12480 | 5' | -54.7 | NC_003324.1 | + | 18375 | 0.68 | 0.627231 |
Target: 5'- aGGCCGgggCAGCGAuugcccGCGCgucggccgauuccgUCAGcGGUCGc -3' miRNA: 3'- -CCGGUa--GUCGCUu-----CGCG--------------AGUU-CCAGC- -5' |
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12480 | 5' | -54.7 | NC_003324.1 | + | 14897 | 0.68 | 0.641562 |
Target: 5'- -cCCGagGGCGAcGCGCUCGAcgcGGUCa -3' miRNA: 3'- ccGGUagUCGCUuCGCGAGUU---CCAGc -5' |
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12480 | 5' | -54.7 | NC_003324.1 | + | 14523 | 0.75 | 0.266128 |
Target: 5'- aGGgCGUCcGCGAcaaggcgcuGGCGCUCAAGGcCGa -3' miRNA: 3'- -CCgGUAGuCGCU---------UCGCGAGUUCCaGC- -5' |
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12480 | 5' | -54.7 | NC_003324.1 | + | 14123 | 0.66 | 0.759883 |
Target: 5'- uGGCUGgaguuuUCGcGCGAGGCGUUUgcGGUCu -3' miRNA: 3'- -CCGGU------AGU-CGCUUCGCGAGuuCCAGc -5' |
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12480 | 5' | -54.7 | NC_003324.1 | + | 14061 | 0.66 | 0.759883 |
Target: 5'- cGCCAUCGcccgauccGCaGAucggcaaagcAGCGCUCAAGGaagCGg -3' miRNA: 3'- cCGGUAGU--------CG-CU----------UCGCGAGUUCCa--GC- -5' |
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12480 | 5' | -54.7 | NC_003324.1 | + | 13180 | 0.74 | 0.317259 |
Target: 5'- -aCCGUCAGCGAGGCuGCgucggCGGcGGUCGg -3' miRNA: 3'- ccGGUAGUCGCUUCG-CGa----GUU-CCAGC- -5' |
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12480 | 5' | -54.7 | NC_003324.1 | + | 12887 | 0.67 | 0.67454 |
Target: 5'- cGCCAUCGuCGAugGGCGCgguguuGGUCGa -3' miRNA: 3'- cCGGUAGUcGCU--UCGCGaguu--CCAGC- -5' |
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12480 | 5' | -54.7 | NC_003324.1 | + | 11220 | 0.78 | 0.169649 |
Target: 5'- cGCCAgacCGGCGAAGCGgacCAAGGUCGg -3' miRNA: 3'- cCGGUa--GUCGCUUCGCga-GUUCCAGC- -5' |
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12480 | 5' | -54.7 | NC_003324.1 | + | 8915 | 0.67 | 0.685466 |
Target: 5'- cGGCCGUguugauagaugCuGCG-AGCG-UCGAGGUCGc -3' miRNA: 3'- -CCGGUA-----------GuCGCuUCGCgAGUUCCAGC- -5' |
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12480 | 5' | -54.7 | NC_003324.1 | + | 6202 | 0.66 | 0.749554 |
Target: 5'- cGCUAUCGGCGuAGGCGC---GGGUa- -3' miRNA: 3'- cCGGUAGUCGC-UUCGCGaguUCCAgc -5' |
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12480 | 5' | -54.7 | NC_003324.1 | + | 3801 | 0.66 | 0.749554 |
Target: 5'- aGCCAuUCAGCGggGauuaCGCUUuuggcGGUCa -3' miRNA: 3'- cCGGU-AGUCGCuuC----GCGAGuu---CCAGc -5' |
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12480 | 5' | -54.7 | NC_003324.1 | + | 2176 | 0.66 | 0.739105 |
Target: 5'- uGCCGaCAGCGcagGAGCuGCUCAcgGGGcCGa -3' miRNA: 3'- cCGGUaGUCGC---UUCG-CGAGU--UCCaGC- -5' |
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12480 | 5' | -54.7 | NC_003324.1 | + | 1742 | 0.67 | 0.685466 |
Target: 5'- cGCCGUCAGCGucagcAGGCGC---AGGcCGa -3' miRNA: 3'- cCGGUAGUCGC-----UUCGCGaguUCCaGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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