Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12483 | 3' | -58.4 | NC_003324.1 | + | 57266 | 0.68 | 0.48691 |
Target: 5'- -aCC-ACGGCGCUUGUCUgugaGCggcagGCCCu -3' miRNA: 3'- ggGGcUGCCGUGAAUAGGg---CGa----CGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 50320 | 0.73 | 0.226764 |
Target: 5'- uCCCUGACGaucGC-CUgAUCgCCGCUGCCUa -3' miRNA: 3'- -GGGGCUGC---CGuGAaUAG-GGCGACGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 49703 | 1.13 | 0.000344 |
Target: 5'- uCCCCGACGGCACUUAUCCCGCUGCCCu -3' miRNA: 3'- -GGGGCUGCCGUGAAUAGGGCGACGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 49087 | 0.66 | 0.558196 |
Target: 5'- -gCCGaaaGCGGCGucUAUUUCGCUGCCg -3' miRNA: 3'- ggGGC---UGCCGUgaAUAGGGCGACGGg -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 46549 | 0.67 | 0.50688 |
Target: 5'- uUCCCG-UGGCugUUcAUCCgGaaGCCCu -3' miRNA: 3'- -GGGGCuGCCGugAA-UAGGgCgaCGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 43145 | 0.68 | 0.448145 |
Target: 5'- uCCUCGACGGCgaGCUUGUUgaGCgagcGCCa -3' miRNA: 3'- -GGGGCUGCCG--UGAAUAGggCGa---CGGg -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 42856 | 0.73 | 0.244563 |
Target: 5'- aCCCGGCcaaGGCACgu-UCuCCGUUGCCg -3' miRNA: 3'- gGGGCUG---CCGUGaauAG-GGCGACGGg -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 42182 | 0.66 | 0.579153 |
Target: 5'- gCCgaaCGACGGCACUgcgCCggaGUgaucgGCCCa -3' miRNA: 3'- -GGg--GCUGCCGUGAauaGGg--CGa----CGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 38442 | 0.68 | 0.478046 |
Target: 5'- uCCCgcaCGACGGCGaccagcgccgcCCCGgUGCCCu -3' miRNA: 3'- -GGG---GCUGCCGUgaaua------GGGCgACGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 34614 | 0.69 | 0.438722 |
Target: 5'- gCUCCGGgaGGCGCUgggcgAUUCCGCUGgCg -3' miRNA: 3'- -GGGGCUg-CCGUGAa----UAGGGCGACgGg -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 32638 | 0.68 | 0.476086 |
Target: 5'- gCUgGAUGGCACgagcgcaacgGagacgaaaagcucUCCCGCUGCCUc -3' miRNA: 3'- gGGgCUGCCGUGaa--------U-------------AGGGCGACGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 29278 | 0.66 | 0.558196 |
Target: 5'- gCUCGAuCGGCGgaUUGcguuccagcUCCUGCUGCUCg -3' miRNA: 3'- gGGGCU-GCCGUg-AAU---------AGGGCGACGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 28589 | 0.66 | 0.568651 |
Target: 5'- uUuuGGCGGUucgcgGCUUccgCCCGCUgucGCCCu -3' miRNA: 3'- gGggCUGCCG-----UGAAua-GGGCGA---CGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 28356 | 0.67 | 0.547797 |
Target: 5'- gCCCGGaacaucguCGGC-CUUGUCCCaaaGCUccaacggauaccGCCCa -3' miRNA: 3'- gGGGCU--------GCCGuGAAUAGGG---CGA------------CGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 26136 | 0.69 | 0.402202 |
Target: 5'- aUCUCGACcGUuCUcGUCCCGCUGCUg -3' miRNA: 3'- -GGGGCUGcCGuGAaUAGGGCGACGGg -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 24668 | 0.66 | 0.589697 |
Target: 5'- gCCCCGAUGauccuGCGCUggucgCCCGCaGCg- -3' miRNA: 3'- -GGGGCUGC-----CGUGAaua--GGGCGaCGgg -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 24490 | 0.72 | 0.27008 |
Target: 5'- gCCCGACGcGCAgUgcauaUCCGCaUGCCCg -3' miRNA: 3'- gGGGCUGC-CGUgAaua--GGGCG-ACGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 21458 | 0.66 | 0.579153 |
Target: 5'- aUCCGGaucuuCGGCAUagagCUCGUUGCCCu -3' miRNA: 3'- gGGGCU-----GCCGUGaauaGGGCGACGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 20670 | 0.7 | 0.365999 |
Target: 5'- --gCGACGGCGCcacagcaccggAUCCCGCUcGCCa -3' miRNA: 3'- gggGCUGCCGUGaa---------UAGGGCGA-CGGg -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 18288 | 0.66 | 0.610876 |
Target: 5'- -gCCGAC-GCGCgggcaaUCGCUGCCCc -3' miRNA: 3'- ggGGCUGcCGUGaauag-GGCGACGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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