Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12484 | 3' | -59.3 | NC_003324.1 | + | 56298 | 0.71 | 0.244886 |
Target: 5'- gGAUGuCGCCGCCGaUCGAgcgauaGGCGAGGc -3' miRNA: 3'- -CUAC-GCGGCGGCcAGCUg-----CCGUUCUu -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 56202 | 0.73 | 0.188913 |
Target: 5'- cGAUGUGUUcCgGGUUGGCGGCGAGAAc -3' miRNA: 3'- -CUACGCGGcGgCCAGCUGCCGUUCUU- -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 55054 | 0.66 | 0.49244 |
Target: 5'- -cUGgGCUGCCG--CGACGGCGcAGAAg -3' miRNA: 3'- cuACgCGGCGGCcaGCUGCCGU-UCUU- -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 54813 | 0.68 | 0.353667 |
Target: 5'- --cGCGCCGUucgCGGUgaGAcCGGCAGGGAg -3' miRNA: 3'- cuaCGCGGCG---GCCAg-CU-GCCGUUCUU- -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 53285 | 0.77 | 0.092935 |
Target: 5'- --cGCGcCCGCCGGUCG-CGGCuuGAGAAa -3' miRNA: 3'- cuaCGC-GGCGGCCAGCuGCCG--UUCUU- -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 50279 | 1.05 | 0.00068 |
Target: 5'- uGAUGCGCCGCCGGUCGACGGCAAGAAg -3' miRNA: 3'- -CUACGCGGCGGCCAGCUGCCGUUCUU- -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 49296 | 0.67 | 0.432704 |
Target: 5'- --gGCGUCGCaggcguuCGGaUCGuCGGCAGGAAg -3' miRNA: 3'- cuaCGCGGCG-------GCC-AGCuGCCGUUCUU- -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 47689 | 0.66 | 0.496492 |
Target: 5'- cAUGCGCgGCgugguaaagcucggaCGGaacuuccggcUCGACGGCGAGGGu -3' miRNA: 3'- cUACGCGgCG---------------GCC----------AGCUGCCGUUCUU- -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 43177 | 0.67 | 0.396798 |
Target: 5'- uAUGC-CCGCUGG-CgGAUGGCGAGAc -3' miRNA: 3'- cUACGcGGCGGCCaG-CUGCCGUUCUu -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 42498 | 0.69 | 0.337367 |
Target: 5'- gGGUGCGCUuCCGGUCcccACGGCAAa-- -3' miRNA: 3'- -CUACGCGGcGGCCAGc--UGCCGUUcuu -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 38356 | 0.68 | 0.361182 |
Target: 5'- cGggGCGgCGCUGGUCGccgucguGCGGgAGGAAg -3' miRNA: 3'- -CuaCGCgGCGGCCAGC-------UGCCgUUCUU- -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 36527 | 0.73 | 0.174458 |
Target: 5'- --aGCGCCGCgGGcgCGACGGCAu--- -3' miRNA: 3'- cuaCGCGGCGgCCa-GCUGCCGUucuu -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 31245 | 0.7 | 0.289649 |
Target: 5'- --aGCGCCGCCGGcCGcacugcgcguuuuuGCGGCGGcGAc -3' miRNA: 3'- cuaCGCGGCGGCCaGC--------------UGCCGUU-CUu -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 30815 | 0.67 | 0.433649 |
Target: 5'- --aGUGCCcaaCGGUCGAUGGCGucGGAc -3' miRNA: 3'- cuaCGCGGcg-GCCAGCUGCCGU--UCUu -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 30062 | 0.66 | 0.48941 |
Target: 5'- ---aCGCCGCCGGUCGcgacaccggccgcuGCGGCu---- -3' miRNA: 3'- cuacGCGGCGGCCAGC--------------UGCCGuucuu -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 24503 | 0.66 | 0.501579 |
Target: 5'- -cUGCGCC-CCGGgcUCGAccaugacauuuucCGGCAGGGu -3' miRNA: 3'- cuACGCGGcGGCC--AGCU-------------GCCGUUCUu -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 20604 | 0.66 | 0.502599 |
Target: 5'- --gGCGCCGUCGccagugacuUCGACGGUAGGc- -3' miRNA: 3'- cuaCGCGGCGGCc--------AGCUGCCGUUCuu -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 15206 | 0.67 | 0.424249 |
Target: 5'- --cGUGgCGCCGuGUCGACGcCGAGAc -3' miRNA: 3'- cuaCGCgGCGGC-CAGCUGCcGUUCUu -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 14665 | 0.74 | 0.160997 |
Target: 5'- -cUGCGCCGCCGGccgcuugCGcCGGCAGGc- -3' miRNA: 3'- cuACGCGGCGGCCa------GCuGCCGUUCuu -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 13167 | 0.68 | 0.370516 |
Target: 5'- -cUGCGUCGgcggCGGUCGGCGGCGGcGGu -3' miRNA: 3'- cuACGCGGCg---GCCAGCUGCCGUU-CUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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