Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12484 | 3' | -59.3 | NC_003324.1 | + | 54813 | 0.68 | 0.353667 |
Target: 5'- --cGCGCCGUucgCGGUgaGAcCGGCAGGGAg -3' miRNA: 3'- cuaCGCGGCG---GCCAg-CU-GCCGUUCUU- -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 55054 | 0.66 | 0.49244 |
Target: 5'- -cUGgGCUGCCG--CGACGGCGcAGAAg -3' miRNA: 3'- cuACgCGGCGGCcaGCUGCCGU-UCUU- -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 56202 | 0.73 | 0.188913 |
Target: 5'- cGAUGUGUUcCgGGUUGGCGGCGAGAAc -3' miRNA: 3'- -CUACGCGGcGgCCAGCUGCCGUUCUU- -5' |
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12484 | 3' | -59.3 | NC_003324.1 | + | 56298 | 0.71 | 0.244886 |
Target: 5'- gGAUGuCGCCGCCGaUCGAgcgauaGGCGAGGc -3' miRNA: 3'- -CUAC-GCGGCGGCcAGCUg-----CCGUUCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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