Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12488 | 5' | -64.1 | NC_003324.1 | + | 57380 | 0.66 | 0.328068 |
Target: 5'- cUCGgCgGAggCGaGGGCGGCacgacCGGCGUUg -3' miRNA: 3'- -GGCgGgCUaaGC-CCCGCCG-----GCCGCAG- -5' |
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12488 | 5' | -64.1 | NC_003324.1 | + | 37151 | 0.66 | 0.334807 |
Target: 5'- gCCGCUCauucgcgGAUUCGGccaaGGuuGGCGUCg -3' miRNA: 3'- -GGCGGG-------CUAAGCCccg-CCggCCGCAG- -5' |
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12488 | 5' | -64.1 | NC_003324.1 | + | 10244 | 0.65 | 0.357998 |
Target: 5'- aCgGgCCGAgaggUCGGGcauugucucGCGGauuuguuCCGGCGUCg -3' miRNA: 3'- -GgCgGGCUa---AGCCC---------CGCC-------GGCCGCAG- -5' |
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12488 | 5' | -64.1 | NC_003324.1 | + | 13180 | 0.72 | 0.139433 |
Target: 5'- aCCGUCagCGAggcugcgUCGGcGGCGGUCGGCGg- -3' miRNA: 3'- -GGCGG--GCUa------AGCC-CCGCCGGCCGCag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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