miRNA: 3'- gGCGGGCUaAGcCC-CGc---CGGCCGCAG- -5'
miRNA: 3'- -GGCGG--GCUa------AGCC-CCGCCGGCCGCag -5'
miRNA: 3'- ggCGGGCU-AAGCCCCGC---------CGG---CCGCAG- -5'
miRNA: 3'- -GGCGGGCUAAGCCCCGCCGGCCGCAG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12488 | 5' | -64.1 | NC_003324.1 | + | 14432 | 0.71 | 0.150608 |
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Target: 5'- aCGCCCGGcUCuGGcGCccauGCCGGCGUCc -3' miRNA: 3'- gGCGGGCUaAGcCC-CGc---CGGCCGCAG- -5' |
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| 12488 | 5' | -64.1 | NC_003324.1 | + | 13180 | 0.72 | 0.139433 |
|
Target: 5'- aCCGUCagCGAggcugcgUCGGcGGCGGUCGGCGg- -3' miRNA: 3'- -GGCGG--GCUa------AGCC-CCGCCGGCCGCag -5' |
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| 12488 | 5' | -64.1 | NC_003324.1 | + | 26005 | 0.73 | 0.101962 |
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Target: 5'- -gGCCCGAcUUCGGGGUGacugagcgcGCCaauGGCGUCa -3' miRNA: 3'- ggCGGGCU-AAGCCCCGC---------CGG---CCGCAG- -5' |
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| 12488 | 5' | -64.1 | NC_003324.1 | + | 52291 | 1.1 | 0.00016 |
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Target: 5'- aCCGCCCGAUUCGGGGCGGCCGGCGUCu -3' miRNA: 3'- -GGCGGGCUAAGCCCCGCCGGCCGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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