miRNA: 3'- uuGAGGGGCCCuGG-------------CGACGGcCCGGa -5'
miRNA: 3'- -UUGAG-GGGCCCugGCGAcGGCCcGGa -5'
miRNA: 3'- -UUGAGGGGCCCUGGCGACGGCCCGGA- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12491 | 3' | -66.4 | NC_003324.1 | + | 20313 | 0.66 | 0.227112 |
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Target: 5'- -uUUCCCCGGG-CUgcauuccaucuggGCUGCC-GGCCa -3' miRNA: 3'- uuGAGGGGCCCuGG-------------CGACGGcCCGGa -5' |
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| 12491 | 3' | -66.4 | NC_003324.1 | + | 42488 | 0.66 | 0.216711 |
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Target: 5'- gAGCUUgCCCGGGugCGCUuCCGGuCCc -3' miRNA: 3'- -UUGAG-GGGCCCugGCGAcGGCCcGGa -5' |
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| 12491 | 3' | -66.4 | NC_003324.1 | + | 54411 | 1.04 | 0.000214 |
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Target: 5'- cAACUCCCCGGGACCGCUGCCGGGCCUc -3' miRNA: 3'- -UUGAGGGGCCCUGGCGACGGCCCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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