Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
12491 | 5' | -56.4 | NC_003324.1 | + | 48982 | 0.66 | 0.676167 |
Target: 5'- cUUGUGGCgGGUGGCGgaguccuGGAuGCGGGcgAAGg -3' miRNA: 3'- -AGUAUCGgCCGCCGC-------UCU-CGCCU--UUC- -5' |
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12491 | 5' | -56.4 | NC_003324.1 | + | 56643 | 0.66 | 0.666394 |
Target: 5'- gUUAUGGgCGGCGGCauGGGucGCGGGc-- -3' miRNA: 3'- -AGUAUCgGCCGCCG--CUCu-CGCCUuuc -5' |
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12491 | 5' | -56.4 | NC_003324.1 | + | 45362 | 0.66 | 0.655506 |
Target: 5'- ---aAGCaGGCGGCGAGcAGC-GAGGGc -3' miRNA: 3'- aguaUCGgCCGCCGCUC-UCGcCUUUC- -5' |
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12491 | 5' | -56.4 | NC_003324.1 | + | 44892 | 0.66 | 0.644597 |
Target: 5'- -gGUAcGCCGGgGcGCGGGGGCuGGAu-- -3' miRNA: 3'- agUAU-CGGCCgC-CGCUCUCG-CCUuuc -5' |
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12491 | 5' | -56.4 | NC_003324.1 | + | 11228 | 0.67 | 0.61184 |
Target: 5'- ---gAGCCGGCcgccagaccGGCGA-AGCGGAccAAGg -3' miRNA: 3'- aguaUCGGCCG---------CCGCUcUCGCCU--UUC- -5' |
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12491 | 5' | -56.4 | NC_003324.1 | + | 36243 | 0.68 | 0.536404 |
Target: 5'- ---aGGUCGGCGGCGuGA-CGGAuGAGa -3' miRNA: 3'- aguaUCGGCCGCCGCuCUcGCCU-UUC- -5' |
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12491 | 5' | -56.4 | NC_003324.1 | + | 45767 | 0.68 | 0.515394 |
Target: 5'- aUCAUGGCCuGCcacgccuuccGC-AGAGCGGAAAGg -3' miRNA: 3'- -AGUAUCGGcCGc---------CGcUCUCGCCUUUC- -5' |
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12491 | 5' | -56.4 | NC_003324.1 | + | 57383 | 0.68 | 0.515394 |
Target: 5'- ---gGGCuCGGCggaGGCGAGGGCGGc--- -3' miRNA: 3'- aguaUCG-GCCG---CCGCUCUCGCCuuuc -5' |
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12491 | 5' | -56.4 | NC_003324.1 | + | 51528 | 0.69 | 0.484533 |
Target: 5'- aCAUGGCCGGUGGCuuGGGCu----- -3' miRNA: 3'- aGUAUCGGCCGCCGcuCUCGccuuuc -5' |
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12491 | 5' | -56.4 | NC_003324.1 | + | 14595 | 0.71 | 0.362673 |
Target: 5'- ---cGGCCGGCGGCGcAG-GCGGu--- -3' miRNA: 3'- aguaUCGGCCGCCGC-UCuCGCCuuuc -5' |
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12491 | 5' | -56.4 | NC_003324.1 | + | 57031 | 0.71 | 0.362673 |
Target: 5'- ---gAG-CGGCGGCGAGGGCGcGGAu- -3' miRNA: 3'- aguaUCgGCCGCCGCUCUCGC-CUUuc -5' |
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12491 | 5' | -56.4 | NC_003324.1 | + | 31315 | 0.72 | 0.314057 |
Target: 5'- ---cGGCCGGCGGCGcuGGcgcuGGCGGggGc -3' miRNA: 3'- aguaUCGGCCGCCGC--UC----UCGCCuuUc -5' |
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12491 | 5' | -56.4 | NC_003324.1 | + | 56195 | 0.78 | 0.135639 |
Target: 5'- uUCcgGGuuGGCGGCGAGAaCGGAAAc -3' miRNA: 3'- -AGuaUCggCCGCCGCUCUcGCCUUUc -5' |
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12491 | 5' | -56.4 | NC_003324.1 | + | 54445 | 1.08 | 0.000909 |
Target: 5'- gUCAUAGCCGGCGGCGAGAGCGGAAAGc -3' miRNA: 3'- -AGUAUCGGCCGCCGCUCUCGCCUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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