Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12492 | 3' | -61.7 | NC_003324.1 | + | 55172 | 1.09 | 0.000215 |
Target: 5'- aCACAGGCCGGAUGGGCUCUCGCCCACg -3' miRNA: 3'- -GUGUCCGGCCUACCCGAGAGCGGGUG- -5' |
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12492 | 3' | -61.7 | NC_003324.1 | + | 28068 | 0.77 | 0.063077 |
Target: 5'- cCACGGcuuGCCGGAUGGGCUCgUGCCUuuGCg -3' miRNA: 3'- -GUGUC---CGGCCUACCCGAGaGCGGG--UG- -5' |
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12492 | 3' | -61.7 | NC_003324.1 | + | 54870 | 0.7 | 0.191584 |
Target: 5'- cCGCAGGcCCGGAU--GCUCgcgaugucggCGCCCGCa -3' miRNA: 3'- -GUGUCC-GGCCUAccCGAGa---------GCGGGUG- -5' |
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12492 | 3' | -61.7 | NC_003324.1 | + | 18516 | 0.68 | 0.293434 |
Target: 5'- gCGCGGGUCGGAUcaucGGCUUccuUCGCCUg- -3' miRNA: 3'- -GUGUCCGGCCUAc---CCGAG---AGCGGGug -5' |
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12492 | 3' | -61.7 | NC_003324.1 | + | 49730 | 0.67 | 0.307864 |
Target: 5'- uGCAGGCgGGAUGGuucaGCgggCGCaCCACc -3' miRNA: 3'- gUGUCCGgCCUACC----CGagaGCG-GGUG- -5' |
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12492 | 3' | -61.7 | NC_003324.1 | + | 32374 | 0.67 | 0.32283 |
Target: 5'- ----aGCCGGugaacuggucGGGCUCUCGCaCCACc -3' miRNA: 3'- gugucCGGCCua--------CCCGAGAGCG-GGUG- -5' |
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12492 | 3' | -61.7 | NC_003324.1 | + | 43246 | 0.67 | 0.338332 |
Target: 5'- gGCuGGCuCGGAUuGGCgCUCGCUCAa -3' miRNA: 3'- gUGuCCG-GCCUAcCCGaGAGCGGGUg -5' |
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12492 | 3' | -61.7 | NC_003324.1 | + | 50814 | 0.66 | 0.379411 |
Target: 5'- aGCAGuGCCaGGAguGGUUCaaCGCCCACa -3' miRNA: 3'- gUGUC-CGG-CCUacCCGAGa-GCGGGUG- -5' |
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12492 | 3' | -61.7 | NC_003324.1 | + | 56630 | 0.66 | 0.388019 |
Target: 5'- gCAUGGGUCGc--GGGCaacUUCGCCCGCa -3' miRNA: 3'- -GUGUCCGGCcuaCCCGa--GAGCGGGUG- -5' |
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12492 | 3' | -61.7 | NC_003324.1 | + | 27901 | 0.66 | 0.370933 |
Target: 5'- aCACcagcGGCUGGAagGGGCUCggCGCCa-- -3' miRNA: 3'- -GUGu---CCGGCCUa-CCCGAGa-GCGGgug -5' |
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12492 | 3' | -61.7 | NC_003324.1 | + | 1814 | 0.66 | 0.362585 |
Target: 5'- -cCAGaCCGGAUc-GCUCUCGUCCACc -3' miRNA: 3'- guGUCcGGCCUAccCGAGAGCGGGUG- -5' |
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12492 | 3' | -61.7 | NC_003324.1 | + | 32815 | 0.66 | 0.354368 |
Target: 5'- aACcGGUCGGc-GGGCUUuguuuUUGCCCACa -3' miRNA: 3'- gUGuCCGGCCuaCCCGAG-----AGCGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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