Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12492 | 5' | -50.7 | NC_003324.1 | + | 15354 | 0.66 | 0.921574 |
Target: 5'- aGGUGCUCGAUGcgcgcgaggacGCUGAauacgagCGCAgcgaGCa -3' miRNA: 3'- aCCACGGGUUAC-----------UGACUa------GCGUaa--CG- -5' |
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12492 | 5' | -50.7 | NC_003324.1 | + | 45960 | 0.66 | 0.908571 |
Target: 5'- aGcGUGCCCGAUGAacaucacGGccUCGCAUcagGCg -3' miRNA: 3'- aC-CACGGGUUACUga-----CU--AGCGUAa--CG- -5' |
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12492 | 5' | -50.7 | NC_003324.1 | + | 23537 | 0.67 | 0.894392 |
Target: 5'- cGGUGCCCcacagcGUGGCggcggGcgCGCucgGCg -3' miRNA: 3'- aCCACGGGu-----UACUGa----CuaGCGuaaCG- -5' |
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12492 | 5' | -50.7 | NC_003324.1 | + | 46155 | 0.67 | 0.894392 |
Target: 5'- gUGGUGCUCAGaagcGACUGGcCGCucaucucgGCa -3' miRNA: 3'- -ACCACGGGUUa---CUGACUaGCGuaa-----CG- -5' |
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12492 | 5' | -50.7 | NC_003324.1 | + | 55428 | 0.67 | 0.894392 |
Target: 5'- cGGUGCCgGuc--UUGAUCGCGUUccaGCg -3' miRNA: 3'- aCCACGGgUuacuGACUAGCGUAA---CG- -5' |
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12492 | 5' | -50.7 | NC_003324.1 | + | 39729 | 0.68 | 0.826774 |
Target: 5'- aGGgcgcGCUCGGUGGCUGGcuaCGCGagcUUGCg -3' miRNA: 3'- aCCa---CGGGUUACUGACUa--GCGU---AACG- -5' |
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12492 | 5' | -50.7 | NC_003324.1 | + | 8244 | 0.69 | 0.807471 |
Target: 5'- aGGgacugGCCCugAAUGGCuaUGAUCGCAgucgaGCg -3' miRNA: 3'- aCCa----CGGG--UUACUG--ACUAGCGUaa---CG- -5' |
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12492 | 5' | -50.7 | NC_003324.1 | + | 8085 | 0.69 | 0.777058 |
Target: 5'- aGGUGaCC-AUGGCUGccacgGUCGcCGUUGCg -3' miRNA: 3'- aCCACgGGuUACUGAC-----UAGC-GUAACG- -5' |
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12492 | 5' | -50.7 | NC_003324.1 | + | 57086 | 0.69 | 0.76658 |
Target: 5'- aGGUGCgCAA-GGCUGGccucgcccUCGCAgcGCa -3' miRNA: 3'- aCCACGgGUUaCUGACU--------AGCGUaaCG- -5' |
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12492 | 5' | -50.7 | NC_003324.1 | + | 22596 | 0.69 | 0.76658 |
Target: 5'- cGGcgGCCUuuUGGCUGccugcCGCGUUGCc -3' miRNA: 3'- aCCa-CGGGuuACUGACua---GCGUAACG- -5' |
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12492 | 5' | -50.7 | NC_003324.1 | + | 7006 | 0.7 | 0.716658 |
Target: 5'- cUGGUGCCCucgGACUGAaccuugauaccgacCGCGgugGCg -3' miRNA: 3'- -ACCACGGGuuaCUGACUa-------------GCGUaa-CG- -5' |
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12492 | 5' | -50.7 | NC_003324.1 | + | 11805 | 0.72 | 0.621425 |
Target: 5'- aUGGUGCCCcgaaGGUGAagaUUGGUgGCGUUGa -3' miRNA: 3'- -ACCACGGG----UUACU---GACUAgCGUAACg -5' |
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12492 | 5' | -50.7 | NC_003324.1 | + | 41813 | 0.77 | 0.360212 |
Target: 5'- gGGUGCC--GUGGCgUGAUCGCAUggaGCg -3' miRNA: 3'- aCCACGGguUACUG-ACUAGCGUAa--CG- -5' |
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12492 | 5' | -50.7 | NC_003324.1 | + | 55208 | 1.12 | 0.001712 |
Target: 5'- aUGGUGCCCAAUGACUGAUCGCAUUGCc -3' miRNA: 3'- -ACCACGGGUUACUGACUAGCGUAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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