Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12493 | 3' | -57.1 | NC_003324.1 | + | 55304 | 1.09 | 0.000578 |
Target: 5'- cCGCGGCUGCUGGAACAAGCUGCCACAg -3' miRNA: 3'- -GCGCCGACGACCUUGUUCGACGGUGU- -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 4712 | 0.76 | 0.136923 |
Target: 5'- gGCGGCUgGCUGGAAaucCAgaaggcGGCUGCUACGg -3' miRNA: 3'- gCGCCGA-CGACCUU---GU------UCGACGGUGU- -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 44903 | 0.73 | 0.235109 |
Target: 5'- gCGCGGggGCUGGAuugcucgaugGCAAGCUG-CGCGg -3' miRNA: 3'- -GCGCCgaCGACCU----------UGUUCGACgGUGU- -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 12892 | 0.71 | 0.311054 |
Target: 5'- uGCGGCgcgucgaaacGCUGGAugGuuuuGCUGCCAg- -3' miRNA: 3'- gCGCCGa---------CGACCUugUu---CGACGGUgu -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 14575 | 0.71 | 0.303439 |
Target: 5'- uCGCGGcCUGCcGGcGCAAGCgGCCGg- -3' miRNA: 3'- -GCGCC-GACGaCCuUGUUCGaCGGUgu -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 54227 | 0.7 | 0.332336 |
Target: 5'- aGCGGCUGCUcaaagcgaaggcgcGGcACcGGUUGCCAUg -3' miRNA: 3'- gCGCCGACGA--------------CCuUGuUCGACGGUGu -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 12189 | 0.69 | 0.413572 |
Target: 5'- uGCGGa--CUGGAACGAGCcGCCGg- -3' miRNA: 3'- gCGCCgacGACCUUGUUCGaCGGUgu -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 23440 | 0.69 | 0.432575 |
Target: 5'- aGUGGCUG-UGGGACAAugacGCccGCCGCGa -3' miRNA: 3'- gCGCCGACgACCUUGUU----CGa-CGGUGU- -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 37994 | 0.68 | 0.462008 |
Target: 5'- gGCGGCcaUGCcGGAGCG-GUUGCgGCGc -3' miRNA: 3'- gCGCCG--ACGaCCUUGUuCGACGgUGU- -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 47528 | 0.68 | 0.482204 |
Target: 5'- -cCGGaCUGCUGcu-CAAGCUGCgGCAg -3' miRNA: 3'- gcGCC-GACGACcuuGUUCGACGgUGU- -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 8428 | 0.67 | 0.534427 |
Target: 5'- cCGcCGGCUGCaGGuGCAGGCggauuUGCgGCGc -3' miRNA: 3'- -GC-GCCGACGaCCuUGUUCG-----ACGgUGU- -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 21355 | 0.67 | 0.534427 |
Target: 5'- uGCGGacCUGCUGGAugAaccgggccuuAGCcucgaucgucUGCCACGu -3' miRNA: 3'- gCGCC--GACGACCUugU----------UCG----------ACGGUGU- -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 38658 | 0.66 | 0.555885 |
Target: 5'- uCGCGGaCUG-UGGAAgGAuauucCUGCCACAu -3' miRNA: 3'- -GCGCC-GACgACCUUgUUc----GACGGUGU- -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 35324 | 0.66 | 0.610455 |
Target: 5'- gCGcCGGCUGCUGcGGuuGCu-GUUGCUGCGg -3' miRNA: 3'- -GC-GCCGACGAC-CU--UGuuCGACGGUGU- -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 9486 | 0.66 | 0.599468 |
Target: 5'- aGCGuGgUGCcaaGAGCAAGCaUGCCACc -3' miRNA: 3'- gCGC-CgACGac-CUUGUUCG-ACGGUGu -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 32748 | 0.66 | 0.588509 |
Target: 5'- uGCGGUcuCUGGAugGAGCccGCaCGCAu -3' miRNA: 3'- gCGCCGacGACCUugUUCGa-CG-GUGU- -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 43632 | 0.66 | 0.577587 |
Target: 5'- gCGCGGCguguggcugaUGCUGGAagccuucaaggACAAGCUuCC-CAa -3' miRNA: 3'- -GCGCCG----------ACGACCU-----------UGUUCGAcGGuGU- -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 35465 | 0.66 | 0.555885 |
Target: 5'- gCGCgGGCgGCc-GAGCAGGCUGCaGCAg -3' miRNA: 3'- -GCG-CCGaCGacCUUGUUCGACGgUGU- -5' |
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12493 | 3' | -57.1 | NC_003324.1 | + | 55045 | 0.66 | 0.555885 |
Target: 5'- gGCGGacgGCUGG-----GCUGCCGCGa -3' miRNA: 3'- gCGCCga-CGACCuuguuCGACGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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