miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12494 3' -57 NC_003324.1 + 15749 0.67 0.547689
Target:  5'- uGGCCCGCCUUGAucgcgucaucgagcGCGccggGGcCGUCGc- -3'
miRNA:   3'- -UCGGGCGGAACU--------------UGUca--CC-GCAGCac -5'
12494 3' -57 NC_003324.1 + 4613 0.68 0.478185
Target:  5'- cGGCUCaUCUUGu-CGGUGGCGUCGa- -3'
miRNA:   3'- -UCGGGcGGAACuuGUCACCGCAGCac -5'
12494 3' -57 NC_003324.1 + 32743 0.7 0.401357
Target:  5'- aAGCCCGCCgaccgGuuUGGUGGCGggcuUUGUGa -3'
miRNA:   3'- -UCGGGCGGaa---CuuGUCACCGC----AGCAC- -5'
12494 3' -57 NC_003324.1 + 30338 0.71 0.341744
Target:  5'- uGGCCCGCaucaucgccgauggUGucgGCGGUGGCGUCGc- -3'
miRNA:   3'- -UCGGGCGga------------ACu--UGUCACCGCAGCac -5'
12494 3' -57 NC_003324.1 + 55414 1.08 0.000848
Target:  5'- aAGCCCGCCUUGAACAGUGGCGUCGUGu -3'
miRNA:   3'- -UCGGGCGGAACUUGUCACCGCAGCAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.