Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12494 | 5' | -53.8 | NC_003324.1 | + | 4644 | 0.66 | 0.796928 |
Target: 5'- gGCcUGgaccuGCGCGCGCUcGAUcacGGCGCg -3' miRNA: 3'- gCGuAC-----UGCGUGCGA-CUAaa-CCGCGa -5' |
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12494 | 5' | -53.8 | NC_003324.1 | + | 14476 | 0.66 | 0.776902 |
Target: 5'- gGCGUu-UGCGCGCUcGGUUgcGGCGCg -3' miRNA: 3'- gCGUAcuGCGUGCGA-CUAAa-CCGCGa -5' |
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12494 | 5' | -53.8 | NC_003324.1 | + | 26803 | 0.66 | 0.766651 |
Target: 5'- aCGCGgccguGCGcUugGCUGGcUUUGGCGCc -3' miRNA: 3'- -GCGUac---UGC-GugCGACU-AAACCGCGa -5' |
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12494 | 5' | -53.8 | NC_003324.1 | + | 37685 | 0.66 | 0.766651 |
Target: 5'- cCGCAUGACgGUGCGUUuGUcauaGGCGCUc -3' miRNA: 3'- -GCGUACUG-CGUGCGAcUAaa--CCGCGA- -5' |
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12494 | 5' | -53.8 | NC_003324.1 | + | 48806 | 0.66 | 0.766651 |
Target: 5'- uGC-UGGCGCcgACGCgg--UUGGCGCc -3' miRNA: 3'- gCGuACUGCG--UGCGacuaAACCGCGa -5' |
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12494 | 5' | -53.8 | NC_003324.1 | + | 21163 | 0.67 | 0.713496 |
Target: 5'- aGCG-GGCGCGCGacuUGAUacGGUGCUg -3' miRNA: 3'- gCGUaCUGCGUGCg--ACUAaaCCGCGA- -5' |
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12494 | 5' | -53.8 | NC_003324.1 | + | 10387 | 0.67 | 0.702568 |
Target: 5'- aGCG-GAUGCACGCggcgGAacguggUGGCGUa -3' miRNA: 3'- gCGUaCUGCGUGCGa---CUaa----ACCGCGa -5' |
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12494 | 5' | -53.8 | NC_003324.1 | + | 8374 | 0.69 | 0.591263 |
Target: 5'- gCGgAUGACGCugGCUGcgacGGCGaCg -3' miRNA: 3'- -GCgUACUGCGugCGACuaaaCCGC-Ga -5' |
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12494 | 5' | -53.8 | NC_003324.1 | + | 39162 | 0.71 | 0.483218 |
Target: 5'- gCGCGcGGCGaCACGUUGAaUUGGCuGCa -3' miRNA: 3'- -GCGUaCUGC-GUGCGACUaAACCG-CGa -5' |
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12494 | 5' | -53.8 | NC_003324.1 | + | 45156 | 0.73 | 0.385578 |
Target: 5'- uGCcUGGCGCguucgaaguaggACGC-GAUUUGGCGCUc -3' miRNA: 3'- gCGuACUGCG------------UGCGaCUAAACCGCGA- -5' |
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12494 | 5' | -53.8 | NC_003324.1 | + | 50194 | 0.75 | 0.301893 |
Target: 5'- gGCAUGGCGC-CGCUGAUaucUGGgGUa -3' miRNA: 3'- gCGUACUGCGuGCGACUAa--ACCgCGa -5' |
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12494 | 5' | -53.8 | NC_003324.1 | + | 55448 | 1.08 | 0.001555 |
Target: 5'- aCGCAUGACGCACGCUGAUUUGGCGCUc -3' miRNA: 3'- -GCGUACUGCGUGCGACUAAACCGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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