Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12495 | 3' | -58.4 | NC_003324.1 | + | 50933 | 0.68 | 0.463354 |
Target: 5'- gCCAuGCUgucgcugGGCGAACCgccugcaGCGAUGuGGGCg -3' miRNA: 3'- -GGUcCGA-------CCGCUUGGg------CGCUAC-CUCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 51157 | 0.66 | 0.576458 |
Target: 5'- uUCAGGCaGGUGAccugccgcgucaACCUGaCGAgcgGGGGUu -3' miRNA: 3'- -GGUCCGaCCGCU------------UGGGC-GCUa--CCUCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 55037 | 0.68 | 0.445124 |
Target: 5'- gCAGGCaaGGCGGACggcugggcugCCGCGAcGGcGCa -3' miRNA: 3'- gGUCCGa-CCGCUUG----------GGCGCUaCCuCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 56071 | 1.12 | 0.000329 |
Target: 5'- uCCAGGCUGGCGAACCCGCGAUGGAGCu -3' miRNA: 3'- -GGUCCGACCGCUUGGGCGCUACCUCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 57040 | 0.66 | 0.576458 |
Target: 5'- -uGGGCUGGgGAGCggCgGCGAgGGcGCg -3' miRNA: 3'- ggUCCGACCgCUUG--GgCGCUaCCuCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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