Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12496 | 3' | -54.6 | NC_003324.1 | + | 53850 | 0.66 | 0.768384 |
Target: 5'- -gGCGUCCGUCCacucgauauugGUgGUUUCaGCCAugAg -3' miRNA: 3'- gaCGUAGGCAGG-----------CA-CGAAG-CGGUugU- -5' |
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12496 | 3' | -54.6 | NC_003324.1 | + | 20884 | 0.66 | 0.737186 |
Target: 5'- -cGCGUCaccaacacgGUCCGccGCcUCGCCAACGu -3' miRNA: 3'- gaCGUAGg--------CAGGCa-CGaAGCGGUUGU- -5' |
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12496 | 3' | -54.6 | NC_003324.1 | + | 34009 | 0.66 | 0.736128 |
Target: 5'- uCUGCAuUCCG-CUGgcaacgcuucuauUGCUUUGUCAGCAa -3' miRNA: 3'- -GACGU-AGGCaGGC-------------ACGAAGCGGUUGU- -5' |
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12496 | 3' | -54.6 | NC_003324.1 | + | 52378 | 0.67 | 0.694142 |
Target: 5'- aUGCAUCCGUCUG-GCcgCGgCAAa- -3' miRNA: 3'- gACGUAGGCAGGCaCGaaGCgGUUgu -5' |
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12496 | 3' | -54.6 | NC_003324.1 | + | 14538 | 0.68 | 0.616896 |
Target: 5'- -aGCAaggUCCG-CCGaUGCUUggaCGCCGGCAu -3' miRNA: 3'- gaCGU---AGGCaGGC-ACGAA---GCGGUUGU- -5' |
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12496 | 3' | -54.6 | NC_003324.1 | + | 45797 | 0.69 | 0.594794 |
Target: 5'- --aCAUCCGaUCCGUGCcgUCGCCu--- -3' miRNA: 3'- gacGUAGGC-AGGCACGa-AGCGGuugu -5' |
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12496 | 3' | -54.6 | NC_003324.1 | + | 42786 | 0.7 | 0.518881 |
Target: 5'- -cGCAU-CGUCCGaugggaugaUGCcUCGCCAGCGg -3' miRNA: 3'- gaCGUAgGCAGGC---------ACGaAGCGGUUGU- -5' |
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12496 | 3' | -54.6 | NC_003324.1 | + | 1883 | 0.7 | 0.508323 |
Target: 5'- -gGgGUCCGUUCGUGCUUC-CgAACGg -3' miRNA: 3'- gaCgUAGGCAGGCACGAAGcGgUUGU- -5' |
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12496 | 3' | -54.6 | NC_003324.1 | + | 54784 | 0.7 | 0.497857 |
Target: 5'- -aGCGUUCGaCCGgauuugGCUUUGCCGACc -3' miRNA: 3'- gaCGUAGGCaGGCa-----CGAAGCGGUUGu -5' |
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12496 | 3' | -54.6 | NC_003324.1 | + | 14666 | 0.71 | 0.437286 |
Target: 5'- cCUGCG-CCG-CCGgccGCUUgCGCCGGCAg -3' miRNA: 3'- -GACGUaGGCaGGCa--CGAA-GCGGUUGU- -5' |
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12496 | 3' | -54.6 | NC_003324.1 | + | 49335 | 0.71 | 0.427603 |
Target: 5'- uCUGCA--CGUgCUGUGCggCGCCAACAg -3' miRNA: 3'- -GACGUagGCA-GGCACGaaGCGGUUGU- -5' |
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12496 | 3' | -54.6 | NC_003324.1 | + | 18138 | 0.73 | 0.372295 |
Target: 5'- gUGUAUUCGacgCCG-GUUUCGCCGACAc -3' miRNA: 3'- gACGUAGGCa--GGCaCGAAGCGGUUGU- -5' |
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12496 | 3' | -54.6 | NC_003324.1 | + | 56240 | 1.09 | 0.001161 |
Target: 5'- cCUGCAUCCGUCCGUGCUUCGCCAACAg -3' miRNA: 3'- -GACGUAGGCAGGCACGAAGCGGUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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