Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12498 | 3' | -57 | NC_003324.1 | + | 22703 | 0.66 | 0.607265 |
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Target: 5'- -aUGCAGCGCGucgcGGGGcUCGGCa--- -3' miRNA: 3'- gaGCGUCGCGUu---CCCCuAGUCGguac -5' |
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| 12498 | 3' | -57 | NC_003324.1 | + | 2837 | 0.66 | 0.596316 |
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Target: 5'- uUCGCcGgGCGAGGGGGagcgucaaCGGCCGa- -3' miRNA: 3'- gAGCGuCgCGUUCCCCUa-------GUCGGUac -5' |
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| 12498 | 3' | -57 | NC_003324.1 | + | 38012 | 0.66 | 0.574519 |
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Target: 5'- gUUGCGGCGCAcGGGGcgC-GUCAa- -3' miRNA: 3'- gAGCGUCGCGUuCCCCuaGuCGGUac -5' |
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| 12498 | 3' | -57 | NC_003324.1 | + | 44100 | 0.66 | 0.574519 |
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Target: 5'- cCUCGCAGCGCucGGcaugaucccGGcggCAGCCGa- -3' miRNA: 3'- -GAGCGUCGCGuuCC---------CCua-GUCGGUac -5' |
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| 12498 | 3' | -57 | NC_003324.1 | + | 37746 | 0.67 | 0.531549 |
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Target: 5'- gUCGCGGCaGCuuGAGcGGGAUC-GCCGa- -3' miRNA: 3'- gAGCGUCG-CG--UUC-CCCUAGuCGGUac -5' |
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| 12498 | 3' | -57 | NC_003324.1 | + | 48314 | 0.67 | 0.519928 |
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Target: 5'- uUCGCAagaagcuGCGCGAGGGGGU-GGCUc-- -3' miRNA: 3'- gAGCGU-------CGCGUUCCCCUAgUCGGuac -5' |
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| 12498 | 3' | -57 | NC_003324.1 | + | 46322 | 0.72 | 0.280105 |
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Target: 5'- gCUCGCgAGCGCGGuGGGcUCuGCCGUGu -3' miRNA: 3'- -GAGCG-UCGCGUUcCCCuAGuCGGUAC- -5' |
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| 12498 | 3' | -57 | NC_003324.1 | + | 57108 | 1.09 | 0.00064 |
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Target: 5'- cCUCGCAGCGCAAGGGGAUCAGCCAUGa -3' miRNA: 3'- -GAGCGUCGCGUUCCCCUAGUCGGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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