miRNA: 3'- gAGUUGCGG---CCAGCACGGCg--G-GAGCG- -5'
miRNA: 3'- gagUUGCGGCCAGCaCGGCG---GGAGCG- -5'
miRNA: 3'- gAGU--UGCGGCC-----AGCACG-GCGGGAGCG- -5'
miRNA: 3'- gAgUUGCG---GCCAGCa---CG-GCGGGAGCG- -5'
miRNA: 3'- -GAGUUGCGGCCAGCACGGCGGGAGCG- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12499 | 5' | -60 | NC_003324.1 | + | 32252 | 0.74 | 0.151172 |
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Target: 5'- gUCAGCGCCcccGGUCGUGUCGggaCgCUCGCc -3' miRNA: 3'- gAGUUGCGG---CCAGCACGGCg--G-GAGCG- -5' |
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| 12499 | 5' | -60 | NC_003324.1 | + | 54594 | 0.75 | 0.135961 |
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Target: 5'- --gAACGCCGGUCGcGCUGCaggCUUCGCg -3' miRNA: 3'- gagUUGCGGCCAGCaCGGCG---GGAGCG- -5' |
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| 12499 | 5' | -60 | NC_003324.1 | + | 49896 | 0.76 | 0.115769 |
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Target: 5'- aUCGcuGCGUCGGcgcgcUCGUGCuCGCCUUCGCu -3' miRNA: 3'- gAGU--UGCGGCC-----AGCACG-GCGGGAGCG- -5' |
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| 12499 | 5' | -60 | NC_003324.1 | + | 20601 | 0.76 | 0.109684 |
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Target: 5'- aUgGGCGCuugCGGUCGgcggGCaCGCCCUCGCu -3' miRNA: 3'- gAgUUGCG---GCCAGCa---CG-GCGGGAGCG- -5' |
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| 12499 | 5' | -60 | NC_003324.1 | + | 57287 | 1.1 | 0.000365 |
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Target: 5'- gCUCAACGCCGGUCGUGCCGCCCUCGCc -3' miRNA: 3'- -GAGUUGCGGCCAGCACGGCGGGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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