Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 125 | 3' | -57.1 | AC_000006.1 | + | 11159 | 1.09 | 0.000332 |
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Target: 5'- gCCUCUGACGGCGCAGCUGUUCCUGGUg -3' miRNA: 3'- -GGAGACUGCCGCGUCGACAAGGACCA- -5' |
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| 125 | 3' | -57.1 | AC_000006.1 | + | 23628 | 0.67 | 0.410615 |
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Target: 5'- gCC-CUGgagcaGCGGCGCAagcucauGCUGgccguggUCCUGGUc -3' miRNA: 3'- -GGaGAC-----UGCCGCGU-------CGACa------AGGACCA- -5' |
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| 125 | 3' | -57.1 | AC_000006.1 | + | 8325 | 0.66 | 0.471223 |
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Target: 5'- gCUCccGGCGGCaGCGGCgGUUCC-GGc -3' miRNA: 3'- gGAGa-CUGCCG-CGUCGaCAAGGaCCa -5' |
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| 125 | 3' | -57.1 | AC_000006.1 | + | 13537 | 0.66 | 0.502651 |
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Target: 5'- cCCUCguacgagagcgUGAUGGCGCAgGCgac-CCUGGa -3' miRNA: 3'- -GGAG-----------ACUGCCGCGU-CGacaaGGACCa -5' |
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| 125 | 3' | -57.1 | AC_000006.1 | + | 1468 | 0.66 | 0.502651 |
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Target: 5'- ---aUGACuaaGCGCAGCUGcgccuaguUUCCUGGg -3' miRNA: 3'- ggagACUGc--CGCGUCGAC--------AAGGACCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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