Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
125 | 5' | -54.7 | AC_000006.1 | + | 10487 | 0.66 | 0.62321 |
Target: 5'- cGCGGCAGaacccgguucgcGGACGGcCGcGGCGagCGGGa -3' miRNA: 3'- -UGUCGUC------------CCUGUU-GCuCCGCaaGUCC- -5' |
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125 | 5' | -54.7 | AC_000006.1 | + | 15028 | 0.66 | 0.62321 |
Target: 5'- gACAGguGcGGCGACG-GGCGU-CAGu -3' miRNA: 3'- -UGUCguCcCUGUUGCuCCGCAaGUCc -5' |
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125 | 5' | -54.7 | AC_000006.1 | + | 9136 | 0.66 | 0.589093 |
Target: 5'- -uGGCGGGGcccgACGACGAcGGCGacgcacCGGGa -3' miRNA: 3'- ugUCGUCCC----UGUUGCU-CCGCaa----GUCC- -5' |
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125 | 5' | -54.7 | AC_000006.1 | + | 24260 | 0.67 | 0.576658 |
Target: 5'- uCAGC-GGGACAAaguCGgaggggaAGGCGUUgCAGGu -3' miRNA: 3'- uGUCGuCCCUGUU---GC-------UCCGCAA-GUCC- -5' |
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125 | 5' | -54.7 | AC_000006.1 | + | 17574 | 0.67 | 0.566526 |
Target: 5'- -gGGCGuGGGugGugGAGGCaggUCAaGGg -3' miRNA: 3'- ugUCGU-CCCugUugCUCCGca-AGU-CC- -5' |
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125 | 5' | -54.7 | AC_000006.1 | + | 16624 | 0.67 | 0.533125 |
Target: 5'- gGCGGCGGGuGGCGgugcgGCGGcGGCGUUUgcuGGc -3' miRNA: 3'- -UGUCGUCC-CUGU-----UGCU-CCGCAAGu--CC- -5' |
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125 | 5' | -54.7 | AC_000006.1 | + | 7048 | 0.68 | 0.522143 |
Target: 5'- uGCGGCcuuGcGGA--GCGAGGUGUgcgUCAGGg -3' miRNA: 3'- -UGUCGu--C-CCUguUGCUCCGCA---AGUCC- -5' |
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125 | 5' | -54.7 | AC_000006.1 | + | 13320 | 0.69 | 0.428031 |
Target: 5'- gGCAGCGgggccugguguGGGACGACGAGGac-UCGGc -3' miRNA: 3'- -UGUCGU-----------CCCUGUUGCUCCgcaAGUCc -5' |
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125 | 5' | -54.7 | AC_000006.1 | + | 24962 | 0.7 | 0.398939 |
Target: 5'- aGCGGCAGGGcuacgaauCAugGAGGCccacaaagCGGGa -3' miRNA: 3'- -UGUCGUCCCu-------GUugCUCCGcaa-----GUCC- -5' |
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125 | 5' | -54.7 | AC_000006.1 | + | 4075 | 0.71 | 0.353362 |
Target: 5'- cAUAGCAGGGGCG-CuGGGCGUggugCuGGa -3' miRNA: 3'- -UGUCGUCCCUGUuGcUCCGCAa---GuCC- -5' |
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125 | 5' | -54.7 | AC_000006.1 | + | 13231 | 0.72 | 0.303702 |
Target: 5'- cGCGGCGGcugcaGCAGCGAGuCGUUCAGGu -3' miRNA: 3'- -UGUCGUCcc---UGUUGCUCcGCAAGUCC- -5' |
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125 | 5' | -54.7 | AC_000006.1 | + | 10840 | 0.72 | 0.291398 |
Target: 5'- cGCAGCGGGGAgGagcccgaggagaugcGCGAcugccgguuucgGGCGggCAGGg -3' miRNA: 3'- -UGUCGUCCCUgU---------------UGCU------------CCGCaaGUCC- -5' |
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125 | 5' | -54.7 | AC_000006.1 | + | 8075 | 0.74 | 0.233174 |
Target: 5'- cGCGGCGGGGGCGgagagcgaagACGAgGGCGcgCAGu -3' miRNA: 3'- -UGUCGUCCCUGU----------UGCU-CCGCaaGUCc -5' |
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125 | 5' | -54.7 | AC_000006.1 | + | 8357 | 0.74 | 0.219035 |
Target: 5'- -gGGCAGGGGCGgcagaggcacgucgGCGuGGCGcUCGGGc -3' miRNA: 3'- ugUCGUCCCUGU--------------UGCuCCGCaAGUCC- -5' |
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125 | 5' | -54.7 | AC_000006.1 | + | 10075 | 0.8 | 0.085809 |
Target: 5'- cCGGCAGcGGugGugGAGGCGcgCGGGa -3' miRNA: 3'- uGUCGUC-CCugUugCUCCGCaaGUCC- -5' |
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125 | 5' | -54.7 | AC_000006.1 | + | 11193 | 1.11 | 0.000461 |
Target: 5'- cACAGCAGGGACAACGAGGCGUUCAGGg -3' miRNA: 3'- -UGUCGUCCCUGUUGCUCCGCAAGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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