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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12503 | 5' | -46.5 | NC_003327.2 | + | 6240 | 0.67 | 0.54985 |
Target: 5'- cCCGgUUGGGCAAGuuucgcgcagggaguGGCAgccccgcagggACGGCCAu- -3' miRNA: 3'- -GGUgAACCUGUUU---------------UCGU-----------UGCUGGUuc -5' |
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12503 | 5' | -46.5 | NC_003327.2 | + | 1317 | 1.13 | 0.000271 |
Target: 5'- gCCACUUGGACAAAAGCAACGACCAAGa -3' miRNA: 3'- -GGUGAACCUGUUUUCGUUGCUGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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