Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12508 | 5' | -57.4 | NC_003345.1 | + | 68488 | 0.66 | 0.737363 |
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Target: 5'- cGCGGGGccaCCCGUggggCGGGUaaucUGCa -3' miRNA: 3'- -CGCCCCaaaGGGCAa---GCCCGgc--AUGa -5' |
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| 12508 | 5' | -57.4 | NC_003345.1 | + | 18148 | 0.66 | 0.717163 |
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Target: 5'- uCGGGaacgUCuCCGUUCGGGCCa---- -3' miRNA: 3'- cGCCCcaa-AG-GGCAAGCCCGGcauga -5' |
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| 12508 | 5' | -57.4 | NC_003345.1 | + | 46080 | 0.67 | 0.686315 |
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Target: 5'- aCGGGGcUUgacCCCGaggaUUGGGCCGgugGCUu -3' miRNA: 3'- cGCCCC-AAa--GGGCa---AGCCCGGCa--UGA- -5' |
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| 12508 | 5' | -57.4 | NC_003345.1 | + | 1439 | 0.67 | 0.665497 |
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Target: 5'- aCGGGGgccgucuccCCCGcuUUCGGGCC-UACa -3' miRNA: 3'- cGCCCCaaa------GGGC--AAGCCCGGcAUGa -5' |
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| 12508 | 5' | -57.4 | NC_003345.1 | + | 75226 | 0.67 | 0.644566 |
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Target: 5'- gGUGGGGcaaUUCCCGcuaUCGGGagCGUugUa -3' miRNA: 3'- -CGCCCCa--AAGGGCa--AGCCCg-GCAugA- -5' |
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| 12508 | 5' | -57.4 | NC_003345.1 | + | 64292 | 0.69 | 0.561092 |
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Target: 5'- aGCGuuGUcgccCCCGUUCGGGCCGgggUGCa -3' miRNA: 3'- -CGCccCAaa--GGGCAAGCCCGGC---AUGa -5' |
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| 12508 | 5' | -57.4 | NC_003345.1 | + | 1383 | 1.09 | 0.001104 |
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Target: 5'- cGCGGGGUUUCCCGUUCGGGCCGUACUu -3' miRNA: 3'- -CGCCCCAAAGGGCAAGCCCGGCAUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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