Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12509 | 3' | -54 | NC_003345.1 | + | 47544 | 0.66 | 0.870506 |
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Target: 5'- gGCUGacgacCUGUUCCGaCGAGUCgCUGAGa -3' miRNA: 3'- -CGACc----GGCGAGGUaGCUCAGaGAUUC- -5' |
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| 12509 | 3' | -54 | NC_003345.1 | + | 48680 | 0.66 | 0.870506 |
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Target: 5'- gGCUGGCCGgggcggUUCAUCGGGUUagUGAc -3' miRNA: 3'- -CGACCGGCg-----AGGUAGCUCAGagAUUc -5' |
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| 12509 | 3' | -54 | NC_003345.1 | + | 14167 | 0.67 | 0.818612 |
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Target: 5'- gGCUGuuccGCCGCacUCCAcgcaguuUCGAGUCUCc--- -3' miRNA: 3'- -CGAC----CGGCG--AGGU-------AGCUCAGAGauuc -5' |
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| 12509 | 3' | -54 | NC_003345.1 | + | 73543 | 0.67 | 0.781591 |
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Target: 5'- --aGGCCGguaucaCUCCAUCGAGUCg----- -3' miRNA: 3'- cgaCCGGC------GAGGUAGCUCAGagauuc -5' |
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| 12509 | 3' | -54 | NC_003345.1 | + | 8069 | 0.69 | 0.699177 |
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Target: 5'- aGUUGGaaCGCUCauuUUGAGUCUUUAAGa -3' miRNA: 3'- -CGACCg-GCGAGgu-AGCUCAGAGAUUC- -5' |
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| 12509 | 3' | -54 | NC_003345.1 | + | 66947 | 0.7 | 0.666922 |
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Target: 5'- --cGGUCGCgUCgGUCGuGUCUCUGAc -3' miRNA: 3'- cgaCCGGCG-AGgUAGCuCAGAGAUUc -5' |
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| 12509 | 3' | -54 | NC_003345.1 | + | 39418 | 0.7 | 0.656093 |
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Target: 5'- --cGG-CGCUCCcUCGGGUgUCUGAGu -3' miRNA: 3'- cgaCCgGCGAGGuAGCUCAgAGAUUC- -5' |
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| 12509 | 3' | -54 | NC_003345.1 | + | 50338 | 0.7 | 0.623516 |
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Target: 5'- uGCUGGCgGCUCCuUCGGGggUCgcGGu -3' miRNA: 3'- -CGACCGgCGAGGuAGCUCagAGauUC- -5' |
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| 12509 | 3' | -54 | NC_003345.1 | + | 3574 | 1.12 | 0.001347 |
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Target: 5'- aGCUGGCCGCUCCAUCGAGUCUCUAAGa -3' miRNA: 3'- -CGACCGGCGAGGUAGCUCAGAGAUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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