Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1251 | 3' | -50.8 | NC_001317.1 | + | 10282 | 0.66 | 0.824026 |
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Target: 5'- aUCAAacGGuGUGCAgC-UCGACGACCaCGg -3' miRNA: 3'- -AGUU--UCuCAUGUgGgAGCUGUUGG-GC- -5' |
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| 1251 | 3' | -50.8 | NC_001317.1 | + | 3978 | 0.66 | 0.824026 |
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Target: 5'- gUCGcAG-GUAuCACCCUCGACG--CCGa -3' miRNA: 3'- -AGUuUCuCAU-GUGGGAGCUGUugGGC- -5' |
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| 1251 | 3' | -50.8 | NC_001317.1 | + | 16013 | 0.66 | 0.803987 |
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Target: 5'- cCGAAuGucGUG-ACCCUCGACAAgCCGa -3' miRNA: 3'- aGUUU-Cu-CAUgUGGGAGCUGUUgGGC- -5' |
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| 1251 | 3' | -50.8 | NC_001317.1 | + | 7250 | 0.67 | 0.772413 |
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Target: 5'- gUCGAugcAGGGcgAUACCCUCGACAcgauuuGCgCCa -3' miRNA: 3'- -AGUU---UCUCa-UGUGGGAGCUGU------UG-GGc -5' |
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| 1251 | 3' | -50.8 | NC_001317.1 | + | 28726 | 0.67 | 0.761538 |
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Target: 5'- aUCGAcGAGaUACACUCU-GACAACCgGc -3' miRNA: 3'- -AGUUuCUC-AUGUGGGAgCUGUUGGgC- -5' |
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| 1251 | 3' | -50.8 | NC_001317.1 | + | 10150 | 0.68 | 0.670243 |
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Target: 5'- -gAAAGcAGaaacccgcauCACCCUCGACAcCCCGg -3' miRNA: 3'- agUUUC-UCau--------GUGGGAGCUGUuGGGC- -5' |
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| 1251 | 3' | -50.8 | NC_001317.1 | + | 1314 | 0.7 | 0.59963 |
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Target: 5'- gCAAAGAGauUGCACCCgcgcUGGCAugCUGc -3' miRNA: 3'- aGUUUCUC--AUGUGGGa---GCUGUugGGC- -5' |
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| 1251 | 3' | -50.8 | NC_001317.1 | + | 5878 | 0.7 | 0.564593 |
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Target: 5'- gCGGAGAGUAaACCCaUGACGAgcCCCGc -3' miRNA: 3'- aGUUUCUCAUgUGGGaGCUGUU--GGGC- -5' |
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| 1251 | 3' | -50.8 | NC_001317.1 | + | 258 | 1.11 | 0.00106 |
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Target: 5'- uUCAAAGAGUACACCCUCGACAACCCGg -3' miRNA: 3'- -AGUUUCUCAUGUGGGAGCUGUUGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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