Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1251 | 5' | -56.3 | NC_001317.1 | + | 221 | 1.04 | 0.000542 |
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Target: 5'- uCUCAAGCCGCCAGCAUGGCGGCUUUUu -3' miRNA: 3'- -GAGUUCGGCGGUCGUACCGCCGAAAA- -5' |
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| 1251 | 5' | -56.3 | NC_001317.1 | + | 166 | 0.77 | 0.068217 |
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Target: 5'- --aAAGCCGCCAuGC-UGGCGGCUUg- -3' miRNA: 3'- gagUUCGGCGGU-CGuACCGCCGAAaa -5' |
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| 1251 | 5' | -56.3 | NC_001317.1 | + | 4431 | 0.73 | 0.136645 |
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Target: 5'- uUCcAGUUcgGCCAGCGUGGCGGCg--- -3' miRNA: 3'- gAGuUCGG--CGGUCGUACCGCCGaaaa -5' |
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| 1251 | 5' | -56.3 | NC_001317.1 | + | 2475 | 0.71 | 0.188227 |
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Target: 5'- gCUCGcGCCGCCGGUGguuucUGGUGGCa--- -3' miRNA: 3'- -GAGUuCGGCGGUCGU-----ACCGCCGaaaa -5' |
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| 1251 | 5' | -56.3 | NC_001317.1 | + | 10530 | 0.7 | 0.242487 |
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Target: 5'- gCUCAGGCUGCaaauCAUGGUGGCg--- -3' miRNA: 3'- -GAGUUCGGCGguc-GUACCGCCGaaaa -5' |
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| 1251 | 5' | -56.3 | NC_001317.1 | + | 7144 | 0.68 | 0.308346 |
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Target: 5'- cCUCAAcGCCGCgauagcgcucauaCAGgGUGGCGGUUg-- -3' miRNA: 3'- -GAGUU-CGGCG-------------GUCgUACCGCCGAaaa -5' |
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| 1251 | 5' | -56.3 | NC_001317.1 | + | 1099 | 0.67 | 0.370079 |
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Target: 5'- -aCGAGCCGCCGGuCAgcuucuccgGGCuGGCa--- -3' miRNA: 3'- gaGUUCGGCGGUC-GUa--------CCG-CCGaaaa -5' |
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| 1251 | 5' | -56.3 | NC_001317.1 | + | 6633 | 0.66 | 0.418355 |
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Target: 5'- gCUUu-GCUGCCGGUuUGGUGGCa--- -3' miRNA: 3'- -GAGuuCGGCGGUCGuACCGCCGaaaa -5' |
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| 1251 | 5' | -56.3 | NC_001317.1 | + | 19095 | 0.66 | 0.418355 |
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Target: 5'- aUCcAGCCGCCGGUgAUGGCugGGUa--- -3' miRNA: 3'- gAGuUCGGCGGUCG-UACCG--CCGaaaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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