Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12510 | 3' | -51.1 | NC_003345.1 | + | 68951 | 0.66 | 0.943531 |
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Target: 5'- --cGUGgaUGAuGCGGUCUUCCGUCu-- -3' miRNA: 3'- uaaCGCa-ACU-UGCCGGAAGGCAGuug -5' |
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| 12510 | 3' | -51.1 | NC_003345.1 | + | 10676 | 0.66 | 0.943531 |
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Target: 5'- -gUGaCGUUGAugGGCgaCUUCCcaaucccgGUCGGCc -3' miRNA: 3'- uaAC-GCAACUugCCG--GAAGG--------CAGUUG- -5' |
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| 12510 | 3' | -51.1 | NC_003345.1 | + | 53103 | 0.66 | 0.938447 |
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Target: 5'- -gUGCuuccUGAACGGUCgucucgCCGUCAAUc -3' miRNA: 3'- uaACGca--ACUUGCCGGaa----GGCAGUUG- -5' |
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| 12510 | 3' | -51.1 | NC_003345.1 | + | 29684 | 0.69 | 0.8196 |
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Target: 5'- --gGCGUUGAcaugaaccgacGCGGCU--UCGUCGGCg -3' miRNA: 3'- uaaCGCAACU-----------UGCCGGaaGGCAGUUG- -5' |
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| 12510 | 3' | -51.1 | NC_003345.1 | + | 53769 | 0.71 | 0.706263 |
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Target: 5'- --gGCGggggUGAACGGCgUUUCCGUCu-- -3' miRNA: 3'- uaaCGCa---ACUUGCCG-GAAGGCAGuug -5' |
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| 12510 | 3' | -51.1 | NC_003345.1 | + | 4731 | 1.09 | 0.003426 |
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Target: 5'- gAUUGCGUUGAACGGCCUUCCGUCAACa -3' miRNA: 3'- -UAACGCAACUUGCCGGAAGGCAGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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