Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
12511 | 3' | -57.4 | NC_003345.1 | + | 30301 | 0.66 | 0.697937 |
Target: 5'- -gCCUaCCG-UGGUGGUUCGcUCGUgGg -3' miRNA: 3'- gaGGAcGGCgACCACCAAGC-AGCAgC- -5' |
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12511 | 3' | -57.4 | NC_003345.1 | + | 63659 | 0.68 | 0.561903 |
Target: 5'- gCUCCUGCCGac-GUGGgaC-UCGUCGg -3' miRNA: 3'- -GAGGACGGCgacCACCaaGcAGCAGC- -5' |
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12511 | 3' | -57.4 | NC_003345.1 | + | 67901 | 0.69 | 0.551587 |
Target: 5'- uUCC-GCCGCUGGa-GUcuuccUCGUCGUCa -3' miRNA: 3'- gAGGaCGGCGACCacCA-----AGCAGCAGc -5' |
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12511 | 3' | -57.4 | NC_003345.1 | + | 5090 | 1.08 | 0.001247 |
Target: 5'- gCUCCUGCCGCUGGUGGUUCGUCGUCGu -3' miRNA: 3'- -GAGGACGGCGACCACCAAGCAGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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