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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12511 | 5' | -50.8 | NC_003345.1 | + | 7476 | 0.66 | 0.951808 |
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Target: 5'- cCUCGGUUGAAUggaacgCCGGucacuCAGGAUa -3' miRNA: 3'- -GAGCCAGCUUAga----GGUCuu---GUCCUAa -5' |
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| 12511 | 5' | -50.8 | NC_003345.1 | + | 46246 | 0.66 | 0.956107 |
|
Target: 5'- gCUCGGU--AAUCUCgGGGAUGGGGa- -3' miRNA: 3'- -GAGCCAgcUUAGAGgUCUUGUCCUaa -5' |
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| 12511 | 5' | -50.8 | NC_003345.1 | + | 17305 | 0.66 | 0.960137 |
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Target: 5'- aCUCGGUCGG--UUCCGGGuggaaauaaGCgAGGAUg -3' miRNA: 3'- -GAGCCAGCUuaGAGGUCU---------UG-UCCUAa -5' |
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| 12511 | 5' | -50.8 | NC_003345.1 | + | 68524 | 0.67 | 0.931845 |
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Target: 5'- -aCGGUCGAuguaUUCCuuGACAGGAUUc -3' miRNA: 3'- gaGCCAGCUua--GAGGucUUGUCCUAA- -5' |
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| 12511 | 5' | -50.8 | NC_003345.1 | + | 42562 | 0.67 | 0.920167 |
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Target: 5'- cCUCGaaGUCGAA-CUUCAGGACAGaGAa- -3' miRNA: 3'- -GAGC--CAGCUUaGAGGUCUUGUC-CUaa -5' |
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| 12511 | 5' | -50.8 | NC_003345.1 | + | 5052 | 1.05 | 0.006995 |
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Target: 5'- cCUCGGUCGAAUCUCCAGAACAGGAUUc -3' miRNA: 3'- -GAGCCAGCUUAGAGGUCUUGUCCUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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